DBT BET Exam Paper Analysis 2025, Check Topic Wise Paper Analysis

The Biotechnology Eligibility Test (DBT BET) 2025 was conducted on 13th May 2025 by the Department of Biotechnology (DBT). This national-level exam serves as a gateway for candidates seeking Junior Research Fellowship (JRF) and admission to Ph.D. programs in biotechnology across India. The exam was held in Computer-Based Test (CBT) mode in English only, with a duration of 3 hours (10:00 AM to 1:00 PM). The paper consisted of 200 multiple-choice questions (MCQS) divided into two sections, totalling 300 marks. Below is a detailed DBT BET Exam Paper Analysis 2025, covering section-wise and topic-wise breakdowns.

DBT JRF Exam Paper Analysis 2025

The DBT BET 2025 exam was conducted smoothly across various test centres. The difficulty level was reported to be moderate, with Section A being slightly easier than Section B. Most questions were concept-based, requiring a strong understanding of fundamental biotechnology topics. The DBT JRF Exam 2025, conducted on 13th May 2025 by the Department of Biotechnology (DBT), was a computer-based test (CBT) featuring 200 MCQs divided into Section A (50 compulsory questions on General Science, Chemistry, Mathematics, and Basic Biotechnology) and Section B (150 questions, attempt 50, focusing on advanced topics like Molecular Biology, Genetics, and Immunology). The exam carried 300 marks with a moderate difficulty level, where Section A was relatively easier (NCERT-based) while Section B demanded in-depth subject knowledge.

DBT BET Exam 2025 Student Feedback

The DBT BET Exam 2024 was conducted successfully, and many aspirants have shared their insights regarding the level of the paper, subject-wise distribution, and the type of questions asked. Based on student feedback, the exam was of easy to moderate difficulty. Below is a detailed version of the exact feedback shared by students:

• A question on Aspergillus niger was asked in the ‘Match the Following’ format.
• Very few questions were asked about Immunology.
• In Enzymes, a question on competitive inhibition was asked. It involved Vmax increasing and Km being asked.
• There were many questions from Biochemistry – around 20 to 30 questions.
• Multiple ‘Match the Following’ type questions were included in the paper.
• The overall difficulty level of the exam was Easy to moderate.
• A large number of questions were asked about Microbiology.

BET Exam analysis – Question Review by Students

The following questions were directly reported by students who appeared for the DBT BET Exam 2025. These reflect the types of conceptual and applied topics that appeared in the exam, especially from sections such as Molecular Biology, Biochemistry, Techniques, and General Science Awareness. The phrasing of these questions represents how candidates recalled them post-exam. This analysis helps aspirants understand the nature of questions and focus areas for future preparation.

• What is the full form of BLAST?
• Where does lipid synthesis occur – in the Golgi apparatus or the endoplasmic reticulum?
• What is the purpose of dideoxynucleotides in Sanger sequencing?
• In Sanger sequencing, do we need to reverse the sequence to obtain the final result?
• Amino acid sequences were provided, and we were asked to identify which one would show the highest absorbance.
• Is paneer a fermented product or a coagulated product?
• Which amino acid is the precursor of vitamin niacin?
• Protein A was a trimer and Protein B was a monomer, both with a subunit molecular weight of 20 kDa. We were asked to determine which protein would elute first and which one would elute later.
• Protein-DNA interaction is not studied in which of the following?
• What was the first transgenic crop?
• A question related to pKa and pH.
• How does ethidium bromide link to DNA?
• In which vessel does bidirectional solute transport occur?
• Calendar-based questions (3 in total).
• Where is white matter located?
• What is the first committed enzyme in glycolysis?
• Match the following: curd, cheese, etc. (Bioprocessing question)
• Which is the weakest acid: acetic acid, HF, HNO₂, or HCN?
• Questions on age and relationships (2–3 questions).
• In Agrobacterium tumefaciens, which two genes activate all others?
• What is the length of an alpha helix with 120 amino acids?
• Do histones contain more arginine and lysine?
• Which amino acids cause fluorescence in proteins? (2 questions)
• Which amino acid contains sulfur?
• Questions on FTIR, UV, and CD spectroscopy (2 questions).
• One question on NMR spectroscopy.
• Match the following: Chromatography principles (2 questions).
• Two questions specifically on gel filtration chromatography.
• If pH is lower than pKa, what is the nature of the ions?
• Given sedimentation coefficients, what is the relation in terms of molecular weight?
• What happens to DNA when grown in N15 for generations and then shifted to N14 for one generation, in terms of the number of bands?
• What do Aspergillus niger, Lactobacillus, Acetobacter, and Saccharomyces produce?
• Which of the following is not a zoonotic disease: cholera, plague, etc.?
• Match the following pathogens with diseases: anthrax, syphilis, leprosy, whooping cough, etc.
• Which technique is used to estimate hCG in a pregnancy test?
• Which monoclonal antibody is not used for cancer treatment?
• How is blood group typing done in laboratories?

DBT Biotechnology Exam Schedule

The Department of Biotechnology (DBT) conducted the Biotechnology Eligibility Test (BET) 2025 on 13th May 2025 in Computer-Based Test (CBT) mode. The exam was held in a single shift from 10:00 AM to 1:00 PM, with candidates required to report early for mandatory verification. Below is the detailed schedule for DBT BET 2025:

Biotechnology Eligibility Test Exam Pattern

The exam pattern for DBT BET 2025 was as follows:

DBT BET Exam Paper Analysis 2025 – Section-wise

The DBT BET 2025 exam featured two distinct sections designed to evaluate both foundational knowledge and specialised expertise in biotechnology. Section A tested general aptitude and basic sciences, while Section B focused on advanced biotechnology concepts. Below is a detailed breakdown of the section-wise difficulty level, key topics, and question distribution:

DBT BET Exam Paper Analysis 2025 – Topic-wise

Section A: Topic-wise Weightage

Section B Topic-wise Weightage

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