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Some properties of enzymes are listed in column X, and their kinetic expressions are listed in column Y. Column X Enzyme properties Column Y Expr
Question

Some properties of enzymes are listed in column X, and their kinetic expressions are listed in column Y.

Column X Enzyme properties

Column Y Expressions

A.

Specific activity

I.

/

B.

Turnover number

II.

Substrate concentration at which v =

C.

Michaelis constant

III.

/ moles of enzyme

D.

Catalytic efficiency

IV.

/ protein concentration

​Which one of the following options represents all correct matches between Column X and Column Y?​​

A.

A-II, B-I, C-III, D-IV

B.

A-III, B-II, C-IV, D-I

C.

A-IV, B-III, C-II, D-I

D.

A-I, B-IV, C-II, D-III

Correct option is C

Let's analyze the given table and match the enzyme properties in Column X with their corresponding kinetic expressions in Column Y.

Step-by-step Matching:

  1. Specific Activity (A)

    • Specific activity is defined as Vmax⁡V_{\max}Vmax per unit of protein concentration.
    • This corresponds to Option (iv): Vmax⁡/protein concentrationV_{\max} / \text{protein concentration}Vmax/protein concentration.
  2. Turnover Number (B)

    • The turnover number (kcatk_{\text{cat}}kcat) represents the number of substrate molecules converted to product per enzyme molecule per second.
    • This corresponds to Option (iii): Vmax⁡V_{\max}Vmax / moles of enzyme.
  3. Michaelis Constant (C)

    • The Michaelis constant (KmK_mKm) is the substrate concentration at which the reaction velocity is half of the maximum velocity (Vmax⁡V_{\max}Vmax).
    • This corresponds to Option (ii): v=(Vmax⁡/2)v = (V_{\max}/2)v=(Vmax/2).
  4. Catalytic Efficiency (D)

    • Catalytic efficiency is represented by the ratio kcat/Kmk_{\text{cat}} / K_mkcat/Km, which measures how efficiently an enzyme converts substrate to product.
    • This corresponds to Option (i): kcat/Kmk_{\text{cat}} / K_mkcat/Km.

Correct Answer:

  • A - iv
  • B - iii
  • C - ii
  • D - i

This matches with Option 3.

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