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Dixon plot is used to study the enzyme inhibition by plotting various expressions of velocity (v) and inhibitor concentration [I] on the X-axis (Colum
Question

Dixon plot is used to study the enzyme inhibition by plotting various expressions of velocity (v) and inhibitor concentration [I] on the X-axis (Column A) and Y-axis (Column B) as given below:

Which one of the following options is the correct combination from columns A and B to draw the Dixon plot?

A.

Column A - iv, Column B - i

B.

Column A - i, Column B - ii

C.

Column A - ii, Column B - iii

D.

Column A - iii, Column B - iv

Correct option is A

Explanation-

A Dixon plot is a graphical method used in enzyme kinetics to determine the type of inhibition an enzyme is experiencing. It is specifically useful when studying how an inhibitor affects enzyme activity.
Axes in a Dixon Plot -                                              

  Axis                                                             What it Represents
X-axis Inhibitor concentration             -                          [I][I]​​
Y-axis Reciprocal of velocity               -                         1v\frac{1}{v}​​

The enzyme's velocity depends on:
               The substrate concentration []
               The inhibitor concentration  []

When you plot 1/v vs. [I], the shape and intersection point of the lines (from multiple fixed
[] values) helps identify the inhibition type:

Types of Inhibition Identified Using Dixon Plot:
                  Inhibition                                                 Type Lines on Dixon Plot Do This
               Competitive                        -                  Lines intersect above the x-axis, on left side
            Non-competitive                    -                  Lines intersect on the x-axis (same Ki)
            Uncompetitive                       -                  Lines are parallel


Final Matching:
X-axis (Column A): iv → [I] (inhibitor conc.)
Y-axis (Column B): i → 1/v (inverse velocity)

So, the correct answer is option a - Column A - iv, Column B - i

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