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CBSE Class 12 Biology Previous Year Question Papers With Solutions

Class 12 Biology Previous Year Question Paper

The Class 12 Biology Previous Year Question Paper will help students to assess their preparation. We have given Class 12 Biology Previous Year Question Paper with solutions on this page to make their preparation easy for CBSE Class 12th Term 2 Examination. The candidates appearing in CBSE Class 12th Term 2 Exam 2022 must solve the Class 12 Biology Previous Year Question Paper given on this page to fetch extra marks. Solve all the Class 12 Biology Previous Year Question Paper given here and bookmark this page to get all the latest updates regarding CBSE Class 12th Term 2 Exam 2022.

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Biology Class 12 Previous Year Question Paper – 2020

Q.1 How do cytokine barriers provide innate immunity in humans?
Ans. Cytokines play a main role in the innate immune response by means of direct mechanisms against the invading agent (inhibiting viral replication) or by activating mechanisms for cells such as macrophages, which upon activation, produce more cytokines.
Q.2 Write the dual purpose served by Deoxyribonucleoside triphosphates in polymerisation?
Ans. Deoxyribonucleoside triphosphates (DNTPs) have dual role in the process of replication.
(i) It acts as substrate.
(ii) It also serves as the source of energy for polymerisation reaction.
Q.3 Write the names of the following:
[a] A 15 mya primate that was ape-like
[b] A 2 mya primate that lived in east African grasslands
Ans. (a) Dryopithecus
(b) Australopithecines
Q.4 Mention the chemical change that proinsulin undergoes, to be able to act as mature insulin.
Ans. In mammals, including humans, insulin is synthesised as a pro-hormone (like a pro-enzyme, the prohormone also needs to be processed before it becomes a fully mature and functional hormone) which contains an extra stretch called the C peptide. This C peptide is not present in the mature insulin and is removed during maturation into insulin.
Q.5 Name two diseases whose spread can be controlled by the eradication of Aedes mosquitoes.
Ans. dengue and chikungunya

Q.6 How did a citizen group called Friends of Arcata Marsh, Arcata, California, USA, help to improve water quality of the marshland using integrated waste water Treatment? Explain in four steps.
Ans. Collaborating with biologists from the Humboldt State University, the townspeople created an integrated waste water treatment process within a natural system.
The cleaning occurs in following stages –
1. The conventional sedimentation, filtering and chlorine treatments are given.
2. After this stage, lots of dangerous pollutants like dissolved heavy metals still remain. To combat this, an innovative approach was taken.
3. The biologists developed a series of six connected marshes over 60 hectares of marshland.
4. Appropriate plants, algae, fungi and bacteria were seeded into this area, which neutralise, absorb and assimilate the pollutants. Hence, as the water flows through the marshes, it gets purified naturally.

Q.7 Your advice is sought to improve the nitrogen content of the soil to be used for cultivation of a nonleguminous terrestrial crop.
[a] Recommend two microbes that can enrich the soil with nitrogen.
[b] Why do leguminous crops not require such enrichment of the soil?
a) Azospirillum andAzotobacter
b) Leguminous plants contain rhizobium bacteria in their root nodules. These bacteria convert
atmospheric nitrogen to a form which can be used by plants. This decreases nitrogen deficiency
in the soil and makes it fertile.
Q.8 You have obtained a high yielding variety of tomato. Name and explain the procedure that ensures retention of the desired characteristics repeatedly in large populations of future generation of the tomato crop.
Ans. Plant breeding is the purposeful manipulation of plant species in order to create desired plant types that are better suited for cultivation, give better yields and are disease resistant.
The main steps in breeding a new genetic variety of a crop are –
Collection of variability, Evaluation and selection of parents, Cross hybridisation among the selected parents, Selection and testing of superior recombinants, Testing, release and commercialisation of new cultivars. Plants, bacteria, fungi and animals whose genes have been altered by manipulation are called
Genetically Modified Organisms. GM plants have been useful in many ways.

Q.9 [a] Name the source plant of heroin drug. How is it obtained from the plant?
[b]Write the effects of heroin on the human body.
a) Heroin commonly called smack is chemically diacetylmorphine which is a white, odourless, bitter crystalline compound. This is obtained by acetylation of morphine, which is extracted from the latex of poppy plant Papaversomniferum.
b) heroin is a depressant and slows down body functions

Q.10 With the help of an algebraic equation, how did hardy-Weinberg explain that in a given
population the frequency of occurrence of alleles of a gene is supposed to remain the same through generations?
Although a prokaryotic cell has no defined nucleus, yet DNA is not scattered throughout the cell . Explain.
Ans. This principle says that allele frequencies in a population are stableand is constant from generation to generation. The gene pool (total gene sand their alleles in a population) remains a constant. This is called genetic equilibrium. Sum total of all the allelic frequencies is 1.
Individual frequencies, for example, can be named p, q, etc. In a diploid, p and q represent the frequency of allele A and allele a. The frequency of AA individuals in a population is simply p2.
This is simply stated in another ways, i.e., the probability that an allele A with a frequency of p
appear on both the chromosomes of a diploid individual is simply the product of the probabilities, i.e., p2. Similarly of aa is q2, of Aa 2pq.

Hence, p2+2pq+q2=1.
This is a binomial expansion of (p+q)2. When frequency measured, differs from expected values, the difference (direction) indicates the extent of evolutionary change. Disturbance in genetic equilibrium, or Hardy- Weinberg equilibrium, i.e., change of frequency of alleles in a population would then be interpreted as resulting in evolution.
In prokaryotes, such as, E. coli, though they do not have a defined nucleus, the DNA is not scattered throughout the cell. DNA (being negatively charged) is held with some proteins (that have positive charges) in a region termed as ‘nucleoid’. The DNA in nucleoid is organised in large loops held by proteins.

Q.11 (a) Differentiate between analogous and homologous structures.
(b) Select and write analogous structures from the list given below:
(i) Wings of butterfly and birds
(ii) Vertebrate hearts
(iii) Tendrils of bougainvillea and cucurbita
(iv) Tubers of sweet potato and potato
a) Homologous structures vs Analogous structures

CBSE Class 12 Biology Previous Year Question Papers With Solutions_40.1

b) Analogous structures:
The term analogous structures is applied in the concept of convergent evolution(convergence),
which pertains to the evolutionary process wherein the organisms evolve bodily parts that are
analogous in terms of structure and function despite their ancestors that are very dissimilar or
(i) Wings of butterfly and birds
(iv) Tubers of sweet potato and potato

Q.12 How has the use of Agrobacterium as vectors helped in controlling Meloidegyneincognitia infestation in tobacco plants? Explain in correct sequence
Ans. Several nematodes parasitise a wide variety of plants and animals including human beings. Anematode Meloidegynein cognitia infects the roots of tobacco plants and causes a great reduction in yield. A novel strategy was adopted to prevent this infestation whichwas based on the process of RNA interference (RNAi). RNAi takes place in all eukaryotic organisms as a method of cellular defense. This method involves:

  • Silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing).
  • The source of this complementary RNA could be from an infection by viruses having RNA
    genomes or mobile genetic elements (transposons)that replicate via an RNA intermediate.
  • Using Agrobacterium vectors,
    1) Nematode-specific genes wereintroduced into the host plant.
    2) The introduction of DNAwas such that it produced both sense and anti-sense RNA in the hostcells. These two RNA’s being complementary to each other formed a doublestranded (dsRNA) that initiated RNAi and thus, silenced the specific mRNAof the nematode.
    3) The consequence was that the parasite could not survivein a transgenic host expressing specific interfering RNA.

Using Agrobacterium vectors,

  • Nematode-specific genes wereintroduced into the host
  • The introduction of DNAwas such that it produced both sense and anti-sense RNA in the These two RNA’s being complementary to each other formed a doublestranded (dsRNA) that initiated RNAi and thus, silenced the specific mRNAof the nematode.
  • The consequence was that the parasite could not survivein a transgenic host expressing specific interfering
  • (a) “India has greater ecosystem diversity than Norway.” Do you agree with the statement ? Give reasons in support of your

(b) Write the difference between genetic biodiversity and species biodiversity that exists at all the levels of biological organisation. 


Explain the effect on the characteristics of a river when urban sewage is discharged into it.

In our biosphere immense diversity (or heterogeneity) exists not only at the species level but at all levels of biological organisation ranging from macromolecules within cells to Biodiversity is the term popularized by the sociobiologist Edward Wilson to describe the combined diversity at all the levels of biological organisation.

Ecological diversity: At the ecosystem level, India, for instance, with its deserts, rain forests, mangroves, coral reefs, wetlands, estuaries, and alpine meadows has greater ecosystem diversity than a Scandinavian country like Norway.

Q. Differences between Genetic Diversity and Species Diversity are given

S. No. Genetic biodiversity Species biodiversity
1. It is related to number of genes and their alleles found in organisms. It is related to number and distribution of species found in an area.
2. It is the trait of the species. It is the trait of the community
3. It influences adaptability and distribution of a species in diverse habitats. It influences biotic interactions and stability of the community.


Effects on characteristics of a river, when urban sewage is discharged:

Solids are relatively easy to remove, but most difficult to remove are dissolved salts such as nitrates, phosphates, and other nutrients, and toxic metal ions and organic compounds.

  • Domestic sewage primarily contains biodegradable organic matter, which readily decomposes – thanks to bacteria and other micro-organisms, which can multiply using these organic substances as substrates and hence utilise some of the components of sewage. It is possible to estimate the amount of biodegradable organic matter in sewage water by measuring Biochemical Oxygen Demand (BOD).
  • Micro-organisms involved in biodegradation of organic matter in the receiving water body consume a lot of oxygen, and as a result there is a sharp decline in dissolved oxygendownstream from the point of sewage This causes mortality of fish and other aquatic creatures.
  • Presence of large amounts of nutrients in waters also causes excessive growth of planktonic (free- floating) algae, called an algal bloom which imparts a distinct colour to the water bodies. Algal blooms cause deterioration of the water quality and fish mortality. Some bloom-forming algae are extremely toxic to human beings and

Q. Explain the mechanism of ‘sex determination’ in birds. How does it differ from that of human beings?

Mechanism of sex determination in birds:-

In birds, a different mechanism of sex determination is observed. In this case the total number of chromosome is same in both males and females. But two different types   of gametes in terms of the sex chromosomes are produced by females, i.e., female heterogamety.

In order to have a distinction with the mechanism of sex determination described earlier, the two different sex chromosomes of a female bird has been designated to be the Z and W chromosomes. In these organisms the females have one Z and one W chromosome, whereas males have a pair of Z- chromosomes besides the autosomes.

But the sex determining mechanism in case of humans is XY type. Out of 23 pairs of chromosomes present, 22 pairs are exactly same in both males and females; these are the autosomes.

A pair of X-chromosomes are present in the female, whereas the presence of an X and Y chromosome are determinant of the male characteristic.

During spermatogenesis among males, 50 per cent of the total sperm produced carry the X- chromosome and the rest 50 per cent has Y-chromosome besides the autosomes.

Females, however, produce only ovum with an X-chromosome. In case the ovum fertilises with a sperm carrying X-chromosome the zygote develops into a female (XX) and the fertilisation of ovum with Y-chromosome carrying sperm results into a male offspring.

Q. Explain out-breeding, out-crossing and cross-breed husbandry.

Ans.       Breeding of animals is an important aspect of animal husbandry. Animal breeding aims at increasing the yield of animals and improving the desirable qualities of the produce.

Out-breeding : Out-breeding is the breeding of the unrelated animals, which may be between individuals of the same breed but having no common ancestors for 4-6 generations (out-crossing) or between different breeds (cross-breeding) or different species (inter-specific hybridisation).

Out-crossing: This is the practice of mating of animals within the same breed, but having no common ancestors on either side of their pedigree up to 4-6 generations. The offspring of such a mating is known as an out-cross. It is the best breeding method for animals that are below average in productivity in milk production, growth rate in beef cattle, etc. A single outcross often helps to overcome inbreeding depression.

Cross-breeding: In this method, superior males of one breed are mated with superior females of another breed. Cross-breeding allows the desirable qualities of two different breeds to be combined. The progeny hybrid animals may themselves be used for commercial production. Alternatively, they may be subjected to some form of inbreeding and selection to develop new stable breeds that may be superior to the existing breeds. Many new animal breeds have been developed by this approach. Hisardale is a new breed of sheep developed in Punjab by crossing Bikaneri ewes and Marino rams.

Q.16 (a) Organic farmers prefer biological control of diseases and pests to the use of chemicals for the same purpose justify?

(b) Give an example of a bacterium, a fungus and an insect that are used as biocontrol agents.

Biological control of pests and diseases: In agriculture, there is a method of controlling pests that relies on natural predation rather than introduced

A key belief of the organic farmer is that biodiversity furthers health. The more variety a landscape has, the more sustainable it is. The organic farmer, therefore, works to create a system where the insects that are sometimes called pests are not eradicated, but instead are kept at manageable levels by a complex system of checks and balances within a living and vibrant ecosystem.

Contrary to the ‘conventional’ farming practices which often use chemical methods to kill both useful and harmful life forms indiscriminately, this is a holistic approach that seeks to develop an understanding of the webs of interaction between the myriad of organisms that constitute the field fauna and flora. The organic farmer holds the view that the eradication of the creatures that are often described as pests is not only possible, but also undesirable, for without them the beneficial predatory and parasitic insects which depend upon them as food or hosts would not be able to survive. Thus, the use of biocontrol measures will greatly reduce our dependence on toxic chemicals and pesticides.

  • Bacteria: Bacillus thuringiensis-to control butterfly caterpillars Fungus: Trichoderma- use in the treatment of plant disease
  • Insect: the Ladybird, and Dragonflies- useful to get rid of aphids and mosquitoes, respectively

Q.17(a) How has the development of bioreactor in biotechnology?

(b) Name the most commonly used bioreactor and describe its working.


  1. Small volume cultures cannot yield appreciable quantities of products to produce in large quantities, the development of bioreactors, where large volumes (100-1000 litres) of culture can be processed, was required. Thus, bioreactors can be thought of as vessels in which raw materials are biologically converted into specific products, individual enzymes, etc., using microbial plant, animal or human cells. A bioreactor provides the optimal conditions for achieving the desired product by providing optimum growth conditions (temperature, pH, substrate, salts, vitamins, oxygen).
  2. The most commonly used bioreactors are of stirring – A stirred-tank reactor is usually cylindrical or with a curved base to facilitate the mixing of the reactor contents. The stirrer facilitates even mixing and oxygen availability throughout the bioreactor. Alternatively air can be bubbled through the reactor. the bioreactor has an agitator system, an oxygen delivery system and a foam control system, a temperature control system, pH control system and sampling ports so that small volumes of the culture can be withdrawn periodically.

Q. 18 Explain the roles of the following with the help of an example each in recombinant DNA technology.

  • Restriction Enzymes
  • Plasmids


Restriction enzymes belong to a larger class of enzymes called These are of two kinds; exonucleases and endonucleases.

Exonucleases remove nucleotides from the ends of the DNA whereas, endonucleases make cuts at specific positions within the DNA. Each restriction endonuclease functions by ‘inspecting’ the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar -phosphate backbones. Each restriction endonuclease recognises a specific palindromic nucleotide sequences in the DNA Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase.

Restriction endonucleases are used in genetic engineering to form ‘recombinant’ molecules of DNA, which are composed of DNA from different sources/genomes.

Plasmids: The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid (autonomously replicating circular extra-chromosomal DNA) of Salmonella typhimurium. Stanley Cohen and Herbert Boyer accomplished this in 1972 by isolating the antibiotic resistance gene by cutting out a piece of DNA from a plasmid which was responsible for conferring antibiotic resistance. The cutting of DNA at specific locations became possible with the discovery of the so-called ‘molecular scissors’– restriction enzymes. The cut piece of DNA was then linked with the plasmid DNA. These plasmid DNA act as vectors to transfer the piece of DNA attached to The linking of antibiotic resistance gene with the plasmid vector became possible with the enzyme DNA ligase, which acts on cut DNA molecules and joins their ends. This makes a new combination of circular autonomously replicating DNA created in vitro and is known as recombinant DNA.

Q. 19 Differentiate between Parthenocarpy and Give one example of each.

Ans. The key difference between parthenogenesis and parthenocarpy is, parthenogenesis is shown by animals and plants while parthenocarpy is shown only by plants.

Parthenogenesis can be defined simply as a reproduction without fertilization. It occurs when a female gamete develops into a new individual without being fertilized by a male gamete.

Parthenogenesis is a normal process seen in many plants, vertebrates, invertebrates, etc.

Parthenocarpy is a process which produces fruits without the fusion of ovule with sperm cell in flowering plants. It occurs due to unsuccessful pollination and fertilization. Also, it can happen due to nonfunctional ovules and sperms. These are the differences between parthenogenesis and parthenocarpy.

Parthenogenesis vs Parthenocarpy
Parthenogenesis is a type of reproduction in which unfertilized egg or ovule is developed into a new organism. Parthenocarpy is a process in which unfertilized ovule is developed into a seedless fruit.
Parthenogenesis produces haploid organisms. Parthenocarpy always produces seedless fruits.
Parthenogenesis is common in plants and animals. Parthenocarpy is common in flowering plants.

Q. 20 Medically it is advised to all young mothers that breastfeeding is the best for their newborn babies. Do you agree? Give reasons in support of your answer.

Ans.       The mammary glands of the female undergo differentiation during pregnancy and starts producing milk towards the end of pregnancy by the process called lactation. This helps the mother in feeding the newborn.

The milk produced during the initial few days of lactation is called colostrums which contains several antibodies absolutely essential to develop resistance for the new-born babies. Breast-feeding during the Initial period of infant growth is recommended by doctors for bringing up a healthy baby.

Q.21 Draw a diagram of a mature human sperm. Label any three parts and write their functions.

It is a microscopic structure composed of a head, neck, a middle piece and a tail.

A plasma membrane envelops the whole body of sperm. The sperm head contains an elongated haploid nucleus, the anterior portion of which is covered by a cap-like structure, acrosome.

The acrosome is filled with enzymes that help fertilisation of the ovum. The middle piece possesses numerous mitochondria, which produce energy for the movement of tail that facilitate sperm motility essential for fertilisation.

The human male ejaculates about 200 to 300 million sperms during coitus of which, for normal fertility, at least 60 per cent sperms must have normal shape and size and at least 40 per cent of them must show vigorous motility.

Q.22 (a) expand VNTR and describe its role in DNA

(b) List any two applications of DNA fingerprinting technique.


VNTR-Variable Number of Tandem Repeats

The VNTR belongs to a class of satellite DNA referred to as mini-satellite.

A small DNA sequence is arranged tandemly in many copy numbers. The copy number varies from chromosome to chromosome in an individual.

The numbers of repeat show very high degree of polymorphism. As a result the size of VNTR varies in size from 0.1 to 20 kb. Consequently, after hybridisation with VNTR probe, the autoradiogram gives many bands of differing sizes. These bands give a characteristic pattern for an individual DNA. It differs from individual to individual in a population except in the case of monozygotic (identical) twins. The sensitivity of the technique has been increased by use of polymerase chain reaction (PCR). Consequently, DNA from a single cell is enough to perform DNA fingerprinting analysis.

In addition to application in forensic science, it has much wider application, such as

    1. In determining population
    2. Genetic diversities

Q.23 Looking at the deteriorating air quality because of air pollution in many cities of the country, the citizens are very much worried and concerned about their health. The doctors have declared health emergency in the cities where the air quality is very severely poor.

(a) Mention any two major causes of air pollution.

b)Write any two harmful effects of air pollution to plants and

c)As a captain of your school Eco-club, suggest any two programmes you would plan to organize in the school so as to bring awareness among the students on how to check air pollution in and around the school.


a) Causes of Air Pollution:

Smokestacks of thermal power plants, smelters and other industries release particulate and gaseous air pollutants together with harmless gases.

Automobiles are a major cause for atmospheric pollution at least in the metro cities.

b) Harmful effects of Air Pollution:

To plants: They reduce growth and yield of crops and cause premature death of plants.

To animals: Air pollutants also deleteriously affect the respiratory system of humans and of animals.

c)As a captain of my school eco-club, I will suggest following 2 programmes for awareness among students:

  1. An assessment should be completed, which would tell us the exact levels of air pollutants that are present in the school’s atmosphere and also what is considered a safe count for those particular

Once the level of exposure has been determined, the next step should be to develop a prevention action plan. This will lower and also prevent the exposure levels in the school area. The prevention techniques will depend on what kinds of pollutants are present. Some prevention guidelines should include:

  • Restricting smoking around the entire school
  • Inspecting building material for harmful substances if construction is taking
  • Installing proper and efficient ventilation systems throughout the school
  1. Once most conventional causes of the air pollution are addressed, the lesser known culprits, such as mold, will remains at large. The reason this goes unnoticed is because this microbe hides in places people generally won’t be looking. Yet this also causes breathing problems and is one of the causes of

Q. 24 Write the scientific name of the organism Thomas Hunt Morgan and his colleagues worked with for their experiments. Explain the correlation between linkage and recombination with respect to genes as studied by them.
(b) How did Sturtevant explain gene mapping while working with Morgan?


(a) State the ‘Central dogma’ as proposed by Francis Crick. Are there any exceptions to it? Support your answer with a reason and an example.

(b) Explain how the biochemical characterization (nature) of “Transforming Principle’ was determined, which was not defined from Griffith’s experiments                   


  1. -Drosophila melanogaster

-Morgan carried out several dihybrid crosses in Drosophila to study genes that were sex-linked. The crosses were similar to the dihybrid crosses carried out by Mendel in peas. For example Morgan hybridised yellow-bodied, white-eyed females to brown-bodied, red-eyed males and intercrossed their F1 progeny. He observed that the two genes did not segregate independently of each other and the F2 ratio deviated very significantly from the 9:3:3:1 ratio (expected when the two genes are independent). Morgan and his group knew that the genes were located on the X chromosome and saw quickly that when the two genes in a dihybrid cross were situated on the same chromosome, the proportion of parental gene combinations were much higher than the non-parental type. Morgan attributed this due to the physical association or linkage of the two genes and coined the term linkage to describe this physical association of genes on a chromosome and the term recombination to describe the generation of non-parental gene combinations.

Morgan and his group also found that even when genes were grouped on the same chromosome, some genes were very tightly linked (showed very low recombination) (Cross A) while others were loosely linked (showed higher recombination) (Cross B).

  1. His student Alfred Sturtevant used the frequency of recombination between gene pairs on the same chromosome as a measure of the distance between genes and ‘mapped’ their position on

the chromosome. Today genetic maps are extensively used as a starting point in the sequencing of whole genomes as was done in the case of the Human Genome Sequencing Project.


a) Central dogma as proposed by Francis Crick:

“DNA makes RNA makes proteins, which in turn facilitate the previous two steps as well as the replication of DNA”, or simply “DNA → RNA → protein”.

Exceptions to the central dogma

The biggest revolution in the central dogma was the discovery of retroviruses, which transcribe RNA into DNA through the use of a special enzyme called reverse transcriptase has resulted in an exception to the central dogma; RNA → DNA → RNA → protein. Also, some virus species are so primitive that they use only RNA → proteins, having not developed DNA. With the discovery of prions, a new exception to the central dogma has been discovered, Protein → Protein. That is, proteins directly replicating themselves by making conformational changes in other proteins. Although retroviruses, certain primitive viruses, and prions may violate the central dogma, they are technically not considered “alive”, and thus the rule that “all cellular life follows the central dogma” still holds true.

b) Biochemical Characterisation of Transforming Principle

 Prior to the work of Oswald Avery, Colin MacLeod and Maclyn McCarty (1933-44), the genetic material was thought to be a protein. They worked to determine the biochemical nature of ‘transforming principle’ in Griffith’s experiment.

They purified biochemicals (proteins, DNA, RNA, etc.) from the heat-killed S cells to see which ones could transform live R cells into S cells. They discovered that DNA alone from S bacteria caused R bacteria to become transformed.

They also discovered that protein-digesting enzymes (proteases) and RNA-digesting enzymes (RNases) did not affect transformation, so the transforming substance was not a protein or RNA. Digestion with DNase did inhibit transformation, suggesting that the DNA caused the transformation. They concluded that DNA is the hereditary material, but not all   biologists were convinced.

Q.25 Following are the responses of different animals to various abiotic factors. Describe each one with the help of an example

  • Regulate
  • Conform
  • Migrate
  • Suspend

(b) If 8 individuals in a population of 80 butterflies die in a week, calculate the death rate of population of butterflies during that period.                    


(a) What is a trophic level in an ecosystem? What is ‘standing crop’ with reference to it?

(b) Explain the role of the ‘first trophic level’ in an ecosystem.  

(c) How is the detritus food chain connected with the grazing food chain in a natural ecosystem?


a)     Responses of different animals to various abiotic factors:

Regulate: Some organisms are able to maintain homeostasis by physiological (sometimes behavioural also) means which ensures constant body temperature, constant osmotic concentration, etc.

All birds and mammals, and a very few lower vertebrate and invertebrate species are indeed capable of such regulation (thermoregulation and osmoregulation). Evolutionary biologists

believe that the ‘success’ of mammals is largely due to their ability to maintain a constant body temperature and thrive whether they live in Antarctica or in the Sahara desert.

Conform: Majority of animals and nearly all plants cannot maintain a constant internal environment. Their body temperature changes with the ambient temperature. In aquatic animals, the osmotic concentration of the body fluids changes with that of the ambient water osmotic concentration.

This is particularly true for small animals like shrews and humming birds. Heat loss or heat gain is a function of surface area. Since small animals have a larger surface area relative to their volume, they tend to lose body heat very fast when it is cold outside; then they have to expend much energy to generate body heat through metabolism.

Migrate: The organism can move away temporarily from the stressful habitat to a more hospitable area and return when stressful period is over. Many animals, particularly birds, during winter undertake long-distance migrations to more hospitable areas. Every winter the famous Keolado National Park (Bharatpur) in Rajasthan host thousands of migratory birds coming from Siberia and other extremely cold northern regions.

Suspend: In bacteria, fungi and lower plants, various kinds of thick walled spores are formed which help them to survive unfavourable conditions.

In higher plants, seeds and some other vegetative reproductive structures serve as means to tide over periods of stress besides helping in dispersal.They do so by reducing their metabolic activity and going into a state of ‘dormancy’.

In animals, the organism, if unable to migrate, might avoid the stress by escaping in time. The familiar case of bears going into hibernation during winter is an example of escape in time.

If 8 individuals in a population of 80 butterflies die in a week,

Death rate in the population during that period is 8/80 = 0.1 individuals per butterfly per week.


  1. Organisms occupy a place in the natural surroundings or in a community according to their feeding relationship with other organisms. Based on the source of their nutrition or food, organisms occupy a specific place in the food chain that is known as their trophic level. Each trophic level has a certain mass of living material at a particular time called as the standing crop. The standing crop is measured as the mass of living organisms (biomass) or the number in a unit area. The biomass of a species is expressed in terms of fresh or dry weight.
  2. Producers belong to the first trophic – The most important trophic level within the ecosystem is the first level: primary producers. This is the basic unit; these organisms can live without feeding off of another level. The only thing that these organisms need to survive is sunlight and water, which they can turn into energy themselves. We as humans as well as the other organisms feed off of this level; we need them to help the other levels produced which in turn help up stay afloat.
  3. The detritus food chain (DFC) begins with dead organic matter. It is made up of decomposers which are heterotrophic organisms, mainly fungi and bacteria. They meet their energy and nutrient requirements by degrading dead organic matter or detritus. These are also known as saprotrophs (sapro: to decompose). Decomposers secrete digestive enzymes that breakdown dead and waste materials into simple, inorganic materials, which are subsequently absorbed by

In an aquatic ecosystem, grazing food chain (GFC) is the major conduit for energy flow. As against this, in a terrestrial ecosystem, a much larger fraction of energy flows through the detritus food chain than through the grazing food chain (GFC). Detritus food chain may be connected with the grazing food chain at some levels: some of the organisms of detritus food chain (DFC) are prey to the grazing food chain (GFC) animals, and in a natural ecosystem, some animals like cockroaches, crows, etc., are omnivores.

Q.26 (a) Describe any two devices in a flowering plant which prevent both autogamy and geitonogamy

b)Explain the events upto double fertilization after the pollen tube enters one of the synergids in an ovule of an angiosperm


 a) Explain menstrual cycle in human females?

b) How can the scientific understanding of the menstrual cycle of human females help as a contraceptive measure?

  • In several species such as papaya, male and female flowers are present on different plants, that is each plant is either male or female (dioecy). This condition prevents both autogamy and Maturity of the stamens and carpels in a bisexual flower is not synchronised (Dichogamy) i.e., male or female flowers mature at different time e.g., Cotton, Ficus etc.
  1. After entering one of the synergids,
  • The pollen tube releases the two male gametes into the cytoplasm of the
  • One of the male gametes moves towards the egg cell and fuses with its nucleus thus completing the
  • This results in the formation of a diploid cell, the
  • The other male gamete moves towards the two polar nuclei located in the central cell and fuses with them to produce a triploid primary endosperm nucleus (PEN).
  • As this involves the fusion of three haploid nuclei it is termed triple
  • Since two types of fusions, syngamy and triple fusion take place in an embryo sac the phenomenon is termed double fertilisation, an event unique to flowering
  • The central cell after triple fusion becomes the primary endosperm cell (PEC) and develops into the endosperm while the zygote develops into an


  1. a) The reproductive cycle in the female primates (e.g. monkeys, apes and human beings) is called menstrual. The first menstruation begins at puberty and is called menarche. In human females, menstruation is repeated at an average interval of about 28/29 days, and the cycle of events starting from one menstruation till the next one is called the menstrual cycle. One ovum is released (ovulation) during the middle of each menstrual cycle.

The cycle starts with the menstrual phase, when menstrual flow occurs and it lasts for 3-5 days. The menstrual flow results due to breakdown of endometrial lining of the uterus and its blood vessels which forms liquid that comes out through vagina. Menstruation only occurs if the released ovum is not fertilised.

The menstrual phase is followed by the follicular phase. During this phase, the primary follicles in the ovary grow to become a fully mature Graafian follicle and simultaneously the endometrium of uterus regenerates through proliferation. These changes in the ovary and the uterus are induced by changes in the levels of pituitary and ovarian hormones. The secretion of gonadotropins (LH and FSH) increases gradually during the follicular phase, and stimulates follicular development as well as secretion of estrogens by the growing follicles. Both LH and FSH attain a peak level in the middle of cycle (about 14 Day)

Rapid secretion of LH leading to its maximum level during the mid-cycle called LH surge induces rupture of Graafian follicle and thereby the release of ovum (ovulation). The ovulation (ovulatory phase) is followed by the luteal phase during which the remaining parts of the Graafian follicle transform as the corpus luteum. The corpus luteum secretes large amounts of progesterone which is essential for maintenance of the endometrium.

In the absence of fertilisation, the corpus luteum degenerates. This causes disintegration of the endometrium leading to menstruation, marking a new cycle. In human beings, menstrual   cycles ceases around 50 years of age; that is termed as menopause. Cyclic menstruation is an indicator of normal reproductive phase and extends between menarche and menopause.

Periodic abstinence is one such method in which the couples avoid or abstain from coitus from day 10 to 17 of the menstrual cycle when ovulation could be expected. As chances of fertilisation are very high during this period, it is called the fertile period. Therefore, by abstaining from coitus during this period, conception could be prevented.

Biology Class 12 Previous Year Question Paper – 2019

Q. British geneticist R.C. Punnett developed a graphical representation of a genetic cross
called “Punnett Square”. Mention the possible result this representation predicts of the
genetic cross carried.
Ans. (Probability of ) all genotypes / genotypic ratio
2. State the two principal outcomes of the experiments conducted by Louis Pasteur on origin
of life.
Ans. Life comes from pre-existing life / biogenesis , dismissed the concept of spontaneous generation
3. Name the layer of the atmosphere that is associated with ‘good ozone’.
Ans. Stratosphere

Mention the term used to describe a population interaction between an orchid growing on
a forest tree.
Ans. Commensalism

4. What are ‘flocs’, formed during secondary treatment of sewage ?
Ans. Masses of bacteria associated with fungal filament (to form mesh like structure)
Write any two places where methanogens can be found.
Ans. Anaerobic sludge (digester) , rumen of cattle / ruminants / stomach of cattle / gut of cattle ,
marshy area , flooded rice fields , biogas plant (Any two) = ½ + ½

5. At what state does the meiosis occur in an organism exhibiting haploidic life cycle and
mention the fate of the products thus produced.
Ans. After zygote formation = ½
haploid organism / haploid spores / (haploid) gametophyte = ½
6. You are conducting artificial hybridization on papaya and potato. Which one of them would
require the step of emasculation and why ? However for both you will use the process of
bagging. Justify giving one reason.
Ans. Potato = 1
Flowers of potato have both male and female reproductive parts in same flower / bisexual flowers/ monoecious plant = ½
Bagging : To prevent unwanted pollens from coming on the stigma = ½
7. How would the gene flow or genetic drift affect the population in which either of them
happen to take place ?
Ans. Results in changed frequency of genes (or alleles) in both populations , causing variation , leading to evolution / speciation / founder effect = 1 + 1
8. Differentiate between the roles of B-lymphocytes and T-lymphocytes in generating immune
Ans. B-lymphocytes : Produce antibodies = 1
T-lymphocytes : Help B-lymphocytes to produce antibodies / kills the pathogen directly
(Killer T-cells)= 1
Principle of vaccination is based on the property of “memory” of the immune system.
Taking one suitable example, justify the statement.
Ans. When a vaccine / heat killed pathogen / attenuated pathogen / weakened pathogen / a preparation of antigenic proteins of pathogen is introduced into the body to prevent chicken pox / measles / any other example it produces antibodies against antigen / pathogen , = 1
It generates B and T memory cells that recognize the pathogen quickly on subsequent exposure , to produce large amount of antibodies which inactivate the pathogen causing the disease(Any other correct example of a disease can also be substituted)
9. Explain the relevance of “Totipotency” and “Somaclones” in raising healthy banana plants
from virus infected banana plants.
Ans. Totipotency : Capacity of (apical / axillary) meristematic tissue of banana plant , which are virus free , to generate whole plant through tissue culture (micropropagation) = ½ × 3
Somaclones : Plants produced are genetically identical to the original plant = ½
10. How is a continuous culture system maintained in bioreactors and why ?
Ans. Used medium is drained out from one side of the bioreactor and fresh medium is added from the other side = 1
This type of culturing method produces a larger biomass leading to higher yields (of desired
protein) = 1
11. List any four ways by which GMO’s have been useful for enhanced crop output.
Ans. Make crops more tolerant to abiotic / cold / heat / drought / salt stresses /
Reduces reliance on chemical pesticides (pest-resistant crops) / Reduce post harvest losses /
Increased efficiency of mineral usage by plant (prevents early exhaustion of soil fertility) / Enhanced nutritional value of food (example vitamin A enriched rice / starch) / To create tailor-made plants
for non food purposes (to supply alternative resources of fuels / pharmaceuticals to industries)
12. Mention four significant services that a healthy forest ecosystem provide.
Ans. Purify air / Production of O2 / Purify water / Mitigate droughts and floods / Nutrient cycling / Generating fertile soils / Provide wildlife habitat / Maintain biodiversity / Pollinate crops / Provide site for carbon storage / Provide aesthetic – cultural – spiritual values / economic benefits / from nature food / industrial products / products of medicinal importance (Any four) OR
Substantiate with the help of one example that in an ecosystem mutualists (i) tend to coevolve and (ii) are also one of the major causes of biodiversity loss.
Ans. Fig species is pollinated only by (its partner) wasp species where the female wasp uses the fruit of fig species as a site for egg laying and nourishing its larvae (mutualists tend to co-evolve / evolution of flower and its pollinated species are tightly linked) / Moth deposits its egg in the locule of the ovary of Yucca plant and the flower in turn gets pollinated by the moth (mutualists tend to coevolve / evolution of flower and its pollinator species are tightly linked) (Any other relevant example explained) = 1
When any one of these two species become extinct – the other species associated with it in obligatory way also becomes extinct and leads to biodiversity loss = 1
13. Pollen banks are playing a very important role in promoting plant breeding programme
the world over. How are pollens preserved in the pollen banks ? Explain. How are such
banks benefitting our farmer ? Write any two ways.
Ans. Cryopreservation / preserved in liquid nitrogen (-196OC) = 1
Availability of pollen of different genetic strains (for wider use) / Cryopreservation increases viability of pollens (which can be used in crop breeding programmes ) / Can be preserved / stored for longer duration / Conserve large number of species / To prevent complete extinction of any species / Maintain biodiversity (Any two) = 1 + 1
14. Draw a labelled diagram to show interrelationship of four accessory ducts in a human
male reproductive system.
Correct diagram with : 1 labelling = ½ , 2 labellings = 1 , 3 labellings = 2 , 4 labellings = 3

Draw a sectional view of the human ovary showing the different stages of developing
follicles, corpus luteum and ovulation.

CBSE Class 12 Biology Previous Year Question Papers With Solutions_50.1
Ovulation/secondary oocyte Secondary follicle /
15. Compare in any three ways the chromosomal theory of inheritance as proposed by Sutton
and Bovery with that of experimental results on pea plant presented by Mendel.
Sutton and Boveri Mendel
1. Chromosomes occur in pairs 1. Factors occur in pairs
2. Chromosomes segregate at 2. Factors segregate at gamete formation
the time of gamete formation stage and only one of each pair is
such that only one of each transmitted to a gamete
pair is transmitted to a gamete
3. Independent pairs of 3. One pair of factors segregate
chromosomes segregate independently of another pairs
independently of each other


(a) Explain linkage and recombination as put forth by T.H. Morgan based on his observations with Drosophila melanogaster crossing experiment.
(b) Write the basis on which Alfred Sturtevant explained gene mapping.
Ans. (a) Linkage : – Physical association of genes on a chromosome ,
– Two genes did not segregate independently of each other
– F2
(phenotypic) ratio deviates (significantly) from 9:3:3:1
Recombination: -Tightly linked genes tend to show fewer recombinant frequency / 1.3%
– Loosely linked genes show higher percentage of recombinant frequency /37.2%
(b) He used the frequency of recombination between gene pairs on the same chromosome as a
measure of distance between genes and mapped their position on the chromosome = 1
16. Explain the mechanism of DNA replication with the help of a replication fork. What role
does the enzyme DNA-ligase play in a DNA replication fork ?
Construct and label a transcription unit from which the RNA segment given below has
been transcribed. Write the complete name of the enzyme that transcribed this RNA.
Ans. Non evaluative , because the choice question is faulty , full marks to be awarded to all
examinees who attempt either of the choice questions.
17. (a) Write two differences between Homo erectus and Homo habilis.
(b) Rearrange the following from early to late geologic periods: Carboniferous, Silurian,
Ans. (a) Homo erectus Homo habilis
(i) Brain capacity 900 cc Brain capacity 650 – 800 cc = 1
(ii) (Probably) ate meat (Probably) did not eat meat = 1
(b) Silurian → Carboniferous → Jurassic = 1
(No mark to be awarded if all the three are not in proper sequence )
18. Name the group of bacteria involved in setting milk into curd. Explain the process they
carry in doing so. Write another beneficial role of such bacteria.
Ans. (group of) LAB / (group of) Lactic acid Bacteria / Lactobacillus species = 1
LAB produce acid that coagulate and partially digest the milk proteins = 1
Increases Vitamin B12 / Checks disease causing microbes in the stomach = 1
19. Bee keeping practice is a good income generating industry. Write the different points to
be kept in mind for successful bee keeping. Write the scientific name of the most common
Indian species used for the purpose.
Knowledge of the nature and habits of bees / selection of suitable location for keeping the
beehive / catching and hiving of swarms (group of bees) / management of beehives during different
seasons / handling and collection of honey and bee wax (Any four) = ½ × 4
– Apis indica = 1
20. (a) Match the microbes listed under Column-A with the products mentioned under ColumnB.
Column – A Column – B
(H) Penicillium notatum (i) Statin
(I) Trichoderma polysporum (ii) ethanol
(J) Monascus purpurea (iii) antibiotic
(K) Saccharomyces cerevisiae (iv) Cyclosporin-A
(b) Why does ‘Swiss Cheese’ develop large holes ?
(a) (H) Penicillium notatum (iii) antibiotic
(I) Trichoderma polysporum (iv) Cyclosporin-A
(J) Monascus purpureus (i) Statin
(K) Saccharomyces cerevisiae (ii) ethanol
(b) Due to production of large amount of CO2
(by Propionibacterium sharmanii) = 1
21. Describe the formation of recombinant DNA by the action of EcoRI.
Ans. EcoRI identifies its palindromic sequence on both vector DNA and foreign DNA / 5’ GAATTC3’, cuts strands of DNA little away from the centre of palindromic sites , but between same two bases (G and A) , this leaves single stranded portion at the end (sticky ends) on each strand , for recombination both vector DNA and foreign DNA , with similar sticky ends are joined by the enzyme DNA ligase
The following diagram can be considered in lieu of the above explanation

CBSE Class 12 Biology Previous Year Question Papers With Solutions_60.1


Describe the process of amplification of “gene of interest” using PCR technique.
Ans. Denaturation of desired DNA into two strands, each acting as templates, for each strands separate set of primer ( two sets of primer) used, with the help of deoxy(ribo)nucleotides and Taq polymerase (DNA polymerase isolated from Thermus aquaticus ), extension of DNA template occurs, resulting in replication of desired DNA (amplification)
The following diagram can be considered in lieu of the above explanation

CBSE Class 12 Biology Previous Year Question Papers With Solutions_70.1

22. Two children, A and B aged 4 and 5 years respectively visited a hospital with a similar
genetic disorder. The girl A was provided enzyme-replacement therapy and was advised
to revisit periodically for further treatment. The girl, B was, however, given a therapy that
did not require revisit for further treatment.
(a) Name the ailments the two girls were suffering from ?
(b) Why did the treatment provided to girl A required repeated visits ?
(c) How was the girl B cured permanently ?
Ans. (a) Adenosine deaminase (ADA) deficiency = 1
(b) (In Enzyme Replacement Therapy) functional ADA is introduced to the patient (by injection),
this therapy is not completely curative / enzyme can act only for a limited time
period = 1 + 1
(c) [As there is no permanent cure at the age of five hence 1 mark of this answer allocated
to part (b)]
23. List six advantages of “ex-situ” approach to conservation of biodiversity.
An endangered / threatened species can be conserved / genetic strains of commercially important plants can be preserved for a long time (seed banks) / biodiversity loss is reduced / gametes of threatened species can be preserved in a viable and fertile condition for long periods (using cryopreservation) / eggs can be fertilized in -vitro / plants can be propagated using tissue culture / economically beneficial / conserve large number of species / aesthetic value = (Any six points)
24. While on a visit to a pond in the city-neighbourhood, the visitors were delighted to find
large expanse of water covered with colourful algal mass.
(a) As a student of biology, do you agree with their delight ? Give reasons in support of
your answer.
(b) Explain the cause of such algal growth.
Ans. (a) No, These algal mass (algal bloom) causes deterioration of the water quality , increase fish mortality , are (extremely) toxic to humans and animals, imparts distinct colour to water bodies
(b) Presence of large amount of nutrients / nitrates and phosphates/ nitrogen and phosphorus in
water body = 1
25. (a) Explain one application of each one of the following :
(A) Amniocentesis
(B) Lactational amenorrhea
(b) Prepare a poster for the school programme depicting the objectives of : “Reproductive and Child Health Care Programme”.
Ans. (a) A. To detect chromosomal disorders / sex determination (legally banned) / detect genetic disorder / Karyotyping = 1
B. To prevent pregnancy / means of natural contraception = 1
C. To assist an infertile couple to have children by tranferring the zygote / early embryo /
embryo at eight blastomere stage into fallopian tube = 1
(b) A poster made on RCH – Any relevant slogan or sketch made should be awarded
marks e.g. Hum Do Hamare Do , Do Boond Zindagi Ke , Beti Bachao Beti Padhao , Stop
STD , Gender selection and detection is punishable,
(Any other relevant theme) = 2
(a) Explain any two ways by which apomictic seed can develop.
(b) List one advantage and one disadvantage of a apomictic crop.
(c) Why do farmers find production of hybrid seeds costly ?
(a) (i) A diploid egg is formed without reduction division which develops into embryo without
fertilization = 1
(ii) Some cells of the nucellus (which are diploid in nature) start dividing and develop into
embryo = 1
(b) Advantage : No segregation of characters in hybrid progeny / Apomictic hybrid can be used
to grow crop year after year / economical as ordinary hybrid seeds are costly = 1
Disadvantage : Can not control deleterious genetic mutation / it reduces genetic diversity from
parents to offspring plants due to lack of variations (in asexual reproduction) / lack ability to
adapt to changing environment = 1
(c) Hybrid seeds are costly as farmers have to purchase seeds year after year /production of
hybrid seeds is a technical and expensive method to be done under controlled conditions = 1
26. Differentiate between incomplete dominance and co-dominance. Substantiate you answer
with one example of each.
Ans. Incomplete Dominance Co-dominance
F1 generation does not resemble Both dominant alleles express themselves F1
either of the parent but show an
intermediate trait
Example : Snapdragon / Antirrhinum sp / Example AB blood group in human = 1
dog flower / Mirabilis jalapa /
Four O’clock plant = 1
Incomplete dominance – When homozygous dominant and homozygous recessive parents are crossed all members of F1 progeny will show intermediate trait = 1
Co dominance – When IA and IB are present together they both produce their own sugar /
antigen = 1
(AB Blood Group) =1
// (Any other suitable cross showing occurance of IA & IB together in offsprings)

(a) Write the contributions of the following scientists in deciphering the genetic code.
Georce Gamow; Hargobind Khorana ; Marshall Nirenberg ; Severo Ochoa
(b) State the importance of a Genetic code in protein biosynthesis.
Ans. (a) George Gamow : Proposed that the Genetic code is constituted of 3 nucleotides / provided proof that the codon is a triplet = 1
Hargobind Khorana : Synthesized RNA molecule with a defined combination of bases
(homopolymers and copolymers) = 1
Marshall Nirenberg : Cell free system for protein synthesis / helped the genetic code to be
deciphered= 1
Severo Ochoa : Described enzyme (Polynucleotide phosphorylase )which polymerises RNA
with defined sequence in a template independent manner (enzymatic synthesis of RNA) = 1
(b) Genetic code – Codes for a specific amino acid which is required for protein synthesis /
provides information about the specific amino acid that form a particular protein /
polypeptide = 1
[4 + 1 = 5 marks]
27. (a) What is “population” according to you as a biology student ?
(b) “The size of a population for any species is not a static parameter.” Justify the
statement with specific reference to fluctuations in the population density of a region
in a given period of time.
Ans. (a) Total number of organisms of a species in a particular area at a particular time = 1
(b) The size of a population for any species is not a static parameter because of the factors like :-
Birth rate/ Natality = ½ , number of births during a given period = ½
Death rate/ Mortality= ½ , number of deaths during a given period= ½
Immigration = ½ , number of individuals of the same species that have come into the habitat
from elsewhere during the time period under consideration= ½
Emigration = ½ , number of individuals of the population who left the habitat and gone
elsewhere during the time period under consideration = ½
(a) What is hydrarch succession ?
(b) Compare the pioneer species and climax communities of hydrarch and xerarch succession respectively.
(c) List the factors upon which the type of invading pioneer species depend in secondary hydrarch succession. Why is the rate of this succession faster than that of primary succession ?
Ans. (a) The gradual and fairly predictable changes in the species composition in a water body /
wetter areas = 1
(b) Hydrarch : Pioneer species – Phytoplanktons = ½
Climax community – Forest / trees = ½
Xerarch : Pioneer species – Lichens = ½
Climax community – Forest / trees = ½
(c) Condition of soil, availability of water, seeds or other propagules = 1
Because (some) soil / sediment is already there, the rate of secondary succession is much
faster than primary succession = 1

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