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​22 transduction was used to map the fadL gene. The result of two-factor crosses between fadL and two linked markers, purF and aroC, are shown below.​
Question

22 transduction was used to map the fadL gene. The result of two-factor crosses between fadL and two linked markers, purF and aroC, are shown below.

Which one of the following is the correct map for the three genes?

A.

B.

C.

D.

Correct option is B

Explanation-

In P22 transduction, genes that are closer together are more likely to be co-transduced (i.e., transferred together).
                               → Higher co-transduction frequency = shorter distance
Given data:
Cross 1 (fadL purF⁺ selected):
                         fadL : 200
                         fadL⁺ : 800
=> Co-transduction frequency = 200 / (200+800) = 20%
Cross 2 (fadL aroC⁺ selected):
                          fadL : 400
                          fadL⁺ : 600
=> Co-transduction frequency = 400 / (400+600) = 40%
Cross 3 (aroC purF⁺ selected):
                          purF : 500
                          purF⁺ : 500
=> Co-transduction frequency = 500 / (500+500) = 50%
Interpretation:
From highest to lowest co-transduction frequency:
                                       aroC–purF = 50% → closest
                                       fadL–aroC = 40%
                                       fadL–purF = 20% → farthest
Conclusion: The correct gene order is - fadL — aroC — purF
This matches Option b
Correct answer: Option b

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