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    ​22 transduction was used to map the fadL gene. The result of two-factor crosses between fadL and two linked markers, purF and aroC, are shown below.​
    Question

    22 transduction was used to map the fadL gene. The result of two-factor crosses between fadL and two linked markers, purF and aroC, are shown below.

    Which one of the following is the correct map for the three genes?

    A.

    B.

    C.

    D.

    Correct option is B

    Explanation-

    In P22 transduction, genes that are closer together are more likely to be co-transduced (i.e., transferred together).
                                   → Higher co-transduction frequency = shorter distance
    Given data:
    Cross 1 (fadL purF⁺ selected):
                             fadL : 200
                             fadL⁺ : 800
    => Co-transduction frequency = 200 / (200+800) = 20%
    Cross 2 (fadL aroC⁺ selected):
                              fadL : 400
                              fadL⁺ : 600
    => Co-transduction frequency = 400 / (400+600) = 40%
    Cross 3 (aroC purF⁺ selected):
                              purF : 500
                              purF⁺ : 500
    => Co-transduction frequency = 500 / (500+500) = 50%
    Interpretation:
    From highest to lowest co-transduction frequency:
                                           aroC–purF = 50% → closest
                                           fadL–aroC = 40%
                                           fadL–purF = 20% → farthest
    Conclusion: The correct gene order is - fadL — aroC — purF
    This matches Option b
    Correct answer: Option b

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