Correct option is A
Explanation-
One-Step Growth Experiment is designed to study bacteriophage replication and involved synchronizing infection of all bacteria at once. After a brief adsorption phase, unadsorbed phages were removed (via dilution or washing). The goal was to observe latent period and burst size — phage production over time.
Option a - "The reproduction of large phage population is synchronized." - CORRECT
This is the essence of the experiment: synchronous infection of bacteria with phages, enabling the study of one full replication cycle.
Option b - "A culture is directly developed by inoculation of single bacterial colony from the agar plate into liquid medium." - INCORRECT
This is a general microbiological practice, not specific to the one-step growth experiment. Ellis and Delbrück started with a liquid culture of E. coli, not a single colony from agar.
Option c - "Involves only a single step of overnight culture development followed by inoculation of a fresh medium with 1% inoculum." - INCORRECT
This sounds more like a routine subculturing method, not related to phage synchrony. The one-step experiment involved infection with phage, not just overnight culturing.
Option d - "Only a single carbon source such as glucose is used in the medium." - INCORRECT
The carbon source is not a defining feature of the experiment. The focus was on phage dynamics, not nutrient composition.
Correct Answer: Option a - The reproduction of large phage population is synchronized.
