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Which of the following statements are incorrect ? (A) Fragments of DNA can be separated by ELISA. (B) Transformation is a procedure through which
Question

Which of the following statements are incorrect ?
(A) Fragments of DNA can be separated by ELISA.
(B) Transformation is a procedure through which a piece of DNA is introduced in a host bacterium.
(C) Recombinant DNA technology does not involve isolation of a desired DNA fragment.
(D) DNA ligases are used for stitching DNA fragments into a vector.
Choose the answer from the options given below :

A.

(A) and (C) only

B.

(A) and (B) only

C.

(B) and (C) only

D.

(A), (C) and (D) only

Correct option is A

The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Recombinant DNA technology involves several steps in specific sequence such as isolation of DNA, fragmentation of DNA by restriction endonucleases, isolation of a desired DNA fragment, ligation of the DNA fragment into a vector, transferring the recombinant DNA into the host, culturing the host cells in a medium at large scale and extraction of the desired product.

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