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    E. coli divides every 20 minutes. If a culture containing 1 × 10⁵ cells is incubated for 100 minutes at 37°C, how many cells would be expected?
    Question

    E. coli divides every 20 minutes. If a culture containing 1 × 10⁵ cells is incubated for 100 minutes at 37°C, how many cells would be expected?

    A.

    32 × 10⁵

    B.

    35 × 10⁵

    C.

    100 × 10⁵

    D.

    5 × 10⁵

    Correct option is A

    Correct Answer: (a)
    Explanation:
    Time = 100 minutes
    Division time = 20 minutes
    Number of divisions = 100 ÷ 20 = 5
    Initial cells = 1 × 10⁵
    After 5 divisions,
    N = N₀ × 2⁵ = 1 × 10⁵ × 32 = 32 × 10⁵ cells.
    Information Booster:
    E. coli doubles every 20 minutes in optimum conditions.
    Bacterial growth is exponential – N = N₀ × 2ⁿ.
    Growth curve has four phases – lag, log, stationary, decline.
    Generation time varies between species and environment.
    Temperature, nutrient supply, and pH affect growth rate.

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