The steps of DNA fingerprinting is as follows:
(i) isolation of DNA
(ii) digestion of DNA by restriction endonucleases
(iii) separation of DNA fragments by electrophoresis
(iv) transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon
(v) hybridisation using labelled VNTR probe
(vi) detection of hybridised DNA fragments by autoradiography
English
50 Questions
200 Marks
45 Mins EnglishE. coli divides every 20 minutes. If a culture containing 1 × 10⁵ cells is incubated for 100 minutes at 37°C, how many cells would be expected?
Which of the following is not a correct statement about Huntington’s disease, an autosomal linked dominant trait in human beings?
In the given two columns below, Column–1 lists the names of scientists and Column–2 lists the scientific concepts described by the scientists.
Match the names of scientists with the scientific concept and choose the correct answer from the options given below.
Column–1 Column–2
(I) Bateson and Punnett (A) Enzyme kinetics
(II) Sutton and Boveri (B) Semi-conservative mode of replication of DNA
(III) Meselson and Stahl (C) Evidence of DNA as genetic material
(IV) Michaelis and Menten (D) Chromosomal basis of inheritance
(V) Hershey and Chase (E) Experiments to prove linkage
Codes:
Which of the following enzyme is responsible for breaking the parental DNA helix ahead of replication fork to swivel the helix?
Which of the following is correct about use of bacterial strain in Avery, Macleod and McCarty experiment to establish DNA as genetic material?
Which of the following is the correct statement about the process of transcription?
Read the following statements and identify the correct one based on codes given below:
(1) Guanine present in DNA is a pyrimidine base.
(2) Deoxyribose sugar of DNA is deoxygenated at 2nd and 3rd carbon atoms.
(3) Nitrogenous base of DNA molecule is bound with 1st carbon atom of deoxyribose sugar.
Codes:
Which of the following fact was not established by the X-ray diffraction crystallography of purified DNA?
