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    Which of the following enzyme is responsible for breaking the parental DNA helix ahead of replication fork to swivel the helix?
    Question

    Which of the following enzyme is responsible for breaking the parental DNA helix ahead of replication fork to swivel the helix?

    A.

    Topoisomerase

    B.

    DNA polymerase I

    C.

    DNA polymerase III

    D.

    Helicase

    Correct option is A

    Correct Answer: (a)
    Explanation:
    Topoisomerase relieves tension and prevents supercoiling by creating temporary breaks and allowing DNA to swivel ahead of the replication fork.
    Information Booster:
    Helicase separates the two strands by breaking H-bonds.
    Topoisomerase prevents overwinding of DNA.
    DNA polymerase III synthesizes new DNA strand.
    DNA polymerase I replaces RNA primers.
    Smooth replication requires coordinated enzyme activity.
    Additional Information (Incorrect Options):
    (b) & (c) are polymerases, not helix-relieving enzymes.
    (d) Helicase unwinds strands but does not relieve torsional strain.

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