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Protein phosphatase 2A (PP2A) is a critical regulator of Cdk1 substrates during the cell cycle. The B55 subunit of PP2A influences the substrate selec
Question

Protein phosphatase 2A (PP2A) is a critical regulator of Cdk1 substrates during the cell cycle. The B55 subunit of PP2A influences the substrate selectivity, localization, and regulation of the enzyme. Given below are a few statements about PP2A and its regulation during the M-phase of the cell cycle.

A. PP2A-B55 activity is high during interphase but inhibited during early mitosis when M-Cdk activity rises.
B. M-Cdk turns off PP2A-B55 via the phosphorylation of an intermediary protein kinase called Greatwall.
C. M-Cdk turns on PP2A-B55 via the phosphorylation of an intermediary protein kinase called Greatwall.
D. When anaphase is initiated and M-Cdk activity declines, PP2A-B55 promotes dephosphorylation of Cdk1 substrates.

Which one of the following combinations represents all the correct statements?

A.

A, B, and D

B.

A, C, and D

C.

C and D only

D.

A and B only

Correct option is A

The question focuses on the regulation of PP2A-B55 during the M-phase of the cell cycle and its role in Cdk1 substrates.

Let's break down the statements:

  • A. PP2A-B55 activity is high during interphase but inhibited during early mitosis when M-Cdk activity rises.

    • Correct. During interphase, PP2A-B55 is active and helps regulate Cdk1 substrates. However, during early mitosis, the activation of M-Cdk (M-phase Cdk) leads to the inhibition of PP2A-B55 to allow for the progression of the cell cycle.
  • B. M-Cdk turns off PP2A-B55 via the phosphorylation of an intermediary protein kinase called Greatwall.

    • Correct. The activation of M-Cdk results in the phosphorylation of Greatwall, which, in turn, inhibits PP2A-B55 activity. This phosphorylation helps to ensure that the cell cycle continues through mitosis by blocking PP2A-B55's regulatory action on Cdk1 substrates.
  • C. M-Cdk turns on PP2A-B55 via the phosphorylation of an intermediary protein kinase called Greatwall.

    • Incorrect. This statement contradicts the previous one. M-Cdk actually turns off PP2A-B55 via the phosphorylation of Greatwall, not activates it.
  • D. When anaphase is initiated and M-Cdk activity declines, PP2A-B55 promotes dephosphorylation of Cdk1 substrates.

    • Correct. Once M-Cdk activity declines after anaphase, PP2A-B55 becomes active again, leading to the dephosphorylation of Cdk1 substrates, helping the cell cycle progress through the later stages of mitosis and exit mitosis.

Correct Combination:

  • A, B, and D are correct statements.
  • C is incorrect because it incorrectly states that M-Cdk activates PP2A-B55 through Greatwall phosphorylation, which is the opposite of what actually happens.

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