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Following statements were made about cell cycle regulation:A.De novo synthesis and destruction of Cyclin B are essential for cell cycle progression in
Question

Following statements were made about cell cycle regulation:

A.De novo synthesis and destruction of Cyclin B are essential for cell cycle progression in yeast.
B.De novo synthesis and destruction of Cyclin B and the related Cyclin dependent Kinase (CDK) are essential for cell cycle progression.
C. CDK activity is regulated by both activating and inhibitory phosphorylation.
D. Retinoblastoma (Rb) functions as an inhibitor of G2 to M transition.
E. Inactivation of Sic 1 is essential for transition into S phase.

Which one of the following represents the combination of the correct statements?

A.

A, C, D

B.

B, C, D

C.

A, C, E

D.

B, C, E

Correct option is C

Explanation-

Statement A: "De novo synthesis and destruction of Cyclin B are essential for cell cycle progression in yeast."
This is mostly true, especially in the context of mitotic control. In yeast, Cyclin B (Clb proteins) is synthesized and degraded to control progression. While Cyclin B requires a CDK (Cdc28 in yeast) to function, this statement may be acceptable if the context assumes CDKs are inherently involved.
​Statement B: "De novo synthesis and destruction of Cyclin B and the related Cyclin dependent Kinase (CDK) are essential for cell cycle progression."

It incorrectly implies that CDKs themselves are also synthesized and degraded de novo during each cycle — this is not true. The error in Statement B lies in suggesting that CDKs undergo the same regulated synthesis/destruction as Cyclins, which is not accurate.
 Statement C: "CDK activity is regulated by both activating and inhibitory phosphorylation."
This is a core regulatory mechanism involving activating phosphorylation by CAK, inhibitory phosphorylation by Wee1 and removal of inhibition by Cdc25 phosphatases.

Statement D:"Retinoblastoma (Rb) functions as an inhibitor of G2 to M transition".
 Rb inhibits G1 to S transition by binding E2F transcription factors. It is phosphorylated by Cyclin D/CDK4/6, releasing E2F and allowing progression into S phase. Not involved in G2 to M transition.
Statement E: "Inactivation of Sic1 is essential for transition into S phase."
In budding yeast, Sic1 inhibits S-phase CDKs. Its phosphorylation and degradation allow DNA replication to start.

Final answer - Option c - A, C and E

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