Correct option is C
Explanation:
The experiment investigates the localization of different proteins in mitochondria using western blotting after treating isolated mitochondria with protease and after incubating them in a hypotonic solution. The proteins being analyzed include:
- Mtg2: A protein of interest whose localization is being determined.
- Cyt c (cytochrome c): Typically found in the intermembrane space of mitochondria.
- Porin: A marker for the outer membrane of mitochondria.
- KDH (alpha-ketoglutarate dehydrogenase): A mitochondrial enzyme located in the matrix.
Untreated Mitochondria
Detection of Proteins:
- All four proteins (Mtg2, Cyt c, Porin, and KDH) are detected.
- The presence of Porin indicates that the outer membrane is intact since Porin is a marker for the outer membrane.
Mitochondria in Hypotonic Solution
Protease Treatment:
- Mitochondria were subjected to protease treatment either directly or after being placed in hypotonic solutions. This can compromise the integrity of the mitochondrial membranes.
Effect of Treatment on Protein Localization and Protection:
- Cyt c is released into the solution, indicating that it was located in the intermembrane space, which is exposed once the outer membrane is compromised.
- KDH remains detectable in the matrix, as it is not accessible to the protease due to its location.
- Mtg2 shows specific patterns that differ based on the treatment, indicating a response to the experimental conditions. If Mtg2 were located in the outer membrane or intermembrane space, it would likely be degraded or released when treated with protease, similar to Cyt c. The detection of Mtg2 in the western blot after hypotonic treatment indicates it is likely in a protected location.
Conclusion:
- Therefore, Mtg2 is localized in the matrix of the mitochondria.
