Correct option is A
Ans. (a) Alexa 488 and Cy5
Alexa 488 and Cy5 are the most suitable fluorochromes for in vivo co-localization studies of two different proteins. These two fluorochromes have distinct excitation and emission spectra, which allows for clear differentiation when visualizing the two proteins. Alexa 488 absorbs light in the blue range and emits green fluorescence, while Cy5 absorbs light in the red range and emits far-red fluorescence. This spectral separation allows for the simultaneous visualization of two proteins in the same sample without spectral overlap, which is critical for accurate co-localization studies.
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Alexa 488: A commonly used green fluorochrome, Alexa 488 has an excitation peak at 495 nm and an emission peak at 519 nm. It is well-suited for visualization of cellular structures and proteins in live cell imaging.
Cy5: Cy5 emits in the far-red region with an excitation peak at 649 nm and emission peak at 667 nm. Its spectral characteristics are well-separated from those of Alexa 488, making it ideal for multiplexing in co-localization experiments.
