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​Which one of the following techniques CANNOT be used for separation, detection, or visualization of DNA?​
Question

Which one of the following techniques CANNOT be used for separation, detection, or visualization of DNA?

A.

Western Blotting

B.

Polyacrylamide gel electrophoresis

C.

Fluorescence microscopy

D.

Denaturing high-performance liquid chromatography

Correct option is A

Explanation-

Option a -  Western Blotting 
This is used for the  detection of proteins, not DNA. It uses antibodies to detect specific proteins after electrophoresis.
Option b - Polyacrylamide Gel Electrophoresis (PAGE) 
PAGE can be used to separate small DNA fragments or oligonucleotides. It is commonly used for DNA sequencing gels.
Option c - Fluorescence Microscopy 
Fluorescent dyes like DAPI or ethidium bromide are used to stain and visualize DNA. It is suitable for DNA visualization.
Option d - Denaturing High-Performance Liquid Chromatography (DHPLC) 
This can be used for mutation detection and separation of DNA fragments based on sequence differences and suitable for DNA.

Correct Answer:
Option 1. Western Blotting
Because it is not used for DNA, it is used for proteins.

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