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    ​Which one of the following techniques CANNOT be used for separation, detection, or visualization of DNA?​
    Question

    Which one of the following techniques CANNOT be used for separation, detection, or visualization of DNA?

    A.

    Western Blotting

    B.

    Polyacrylamide gel electrophoresis

    C.

    Fluorescence microscopy

    D.

    Denaturing high-performance liquid chromatography

    Correct option is A

    Explanation-

    Option a -  Western Blotting 
    This is used for the  detection of proteins, not DNA. It uses antibodies to detect specific proteins after electrophoresis.
    Option b - Polyacrylamide Gel Electrophoresis (PAGE) 
    PAGE can be used to separate small DNA fragments or oligonucleotides. It is commonly used for DNA sequencing gels.
    Option c - Fluorescence Microscopy 
    Fluorescent dyes like DAPI or ethidium bromide are used to stain and visualize DNA. It is suitable for DNA visualization.
    Option d - Denaturing High-Performance Liquid Chromatography (DHPLC) 
    This can be used for mutation detection and separation of DNA fragments based on sequence differences and suitable for DNA.

    Correct Answer:
    Option 1. Western Blotting
    Because it is not used for DNA, it is used for proteins.

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