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​The Green Fluorescent Protein (GFP) from the deep-sea jellyfish Aequorea victoria has excitation peaks at 395 nm and 475 nm. Its emission peak is at
Question

The Green Fluorescent Protein (GFP) from the deep-sea jellyfish Aequorea victoria has excitation peaks at 395 nm and 475 nm. Its emission peak is at 509 nm, which is in the green portion of the visible spectrum. In the deep-sea habitat of this marine organism, what is the source of light for excitation of GFP?

A.

Blue light emitted from the oxidation of the cofactor coelenterazine as energy transferred to GFP.

B.

Blue light emitted by aequorin present in A. victoria is absorbed by GFP.

C.

Light emitted by other organisms in ocean seabed is absorbed by GFP in jellyfish.

D.

Ocean currents provide electrical energy that is converted to light.

Correct option is A

Explanation-

The Green Fluorescent Protein (GFP) found in the jellyfish Aequorea victoria does not fluoresce on its own. It requires excitation by blue light, which in this organism is generated internally. Aequorin, a photoprotein also present in Aequorea victoria, emits blue light when it reacts with calcium ions. This blue light (around 470 nm) is then absorbed by GFP, exciting it and causing GFP to emit green fluorescence (509 nm).
Therefore:
Option a is  Correct - Blue-light emitted from the oxidation of the cofactor coelenterazine is energy transferred to GFP.
This describes the correct bioluminescent mechanism via aequorin and coelenterazine.


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