Correct option is B
Explanation-
Option a - Structured illumination microscope (SIM): INCORRECT
It increases resolution using patterned light. It does not require photoactivatable probes.
Option b - dSTORM (direct Stochastic Optical Reconstruction Microscopy): CORRECT
It is a type of single-molecule localization microscopy (SMLM). It requires fluorophores that can switch between bright and dark states, often achieved via photoactivation or photoswitching. This is one of the techniques that directly uses photoactivatable probes for achieving super-resolution.
Option c - Stimulated emission depletion microscopy (STED): INCORRECT
It uses a depletion laser to switch off fluorescence at the periphery, narrowing the point spread function. It does not require photoactivatable probes.
Option d - Laser scanning confocal microscope: INCORRECT
It uses pinhole and point-scanning for better resolution than widefield, but not super-resolution. It does not use photoactivatable probes.
So, the correct answer is Option b — dSTORM (direct Stochastic Optical Reconstruction Microscopy)
