Correct option is B
In animal cell culture, trypsin is an enzyme used to detach adherent cells from the surface of the culture dish during passaging. Cells that grow attached to the substrate (adherent cells) require trypsin for passaging.
Hematopoietic cells, however, are suspension cells, meaning they naturally float in the culture medium and do not adhere to the surface. Because of this, they do not require trypsin for passaging. Instead, they can be simply transferred from one culture vessel to another using pipetting.
Information Booster:
- Trypsin is a proteolytic enzyme used to break cell-substrate adhesion in adherent cultures.
- Adherent cells include epithelial cells, fibroblasts, and myoblasts, which require trypsin for passaging.
- Suspension cells, such as hematopoietic cells and some immune cells, do not require trypsin as they do not adhere to the culture dish.
- Passaging (subculturing) is necessary to maintain cell viability and prevent overgrowth.
- Other dissociation agents used in cell culture include EDTA, collagenase, and Accutase.
Additional Knowledge:
(a) Epithelial cells:
- Adherent cells that require attachment to a substrate for growth.
- Need trypsin treatment to detach from the surface for passaging.
- Examples: Kidney epithelial cells, lung epithelial cells.
(b) Hematopoietic cells (Correct Answer):
- Suspension cells that naturally float in the medium.
- Do not require trypsin for passaging.
- Examples: Bone marrow cells, blood cells, lymphocytes.
(c) Fibroblasts:
- Adherent cells that attach to the culture dish.
- Require trypsin for passaging.
- Examples: Connective tissue fibroblasts.
(d) Myoblasts:
- Adherent muscle precursor cells that attach to the substrate.
- Require trypsin for detachment.
- Examples: Skeletal muscle myoblasts.
