Correct option is C
The correct answer is (3) because Phage M13 does not have a RNA genome. Phage M13 has a single-stranded circular DNA genome, not RNA. M13 is a filamentous bacteriophage that infects E. coli and is often used in molecular biology for cloning and phage display technologies.
Explanation of the Other Options:
- (1) Phage Mu is used to create insertion mutations: Correct. Phage Mu is a transposon used in genetic studies to create insertion mutations in the genome. It is used to study mutagenesis and gene function.
- (2) Phage P1 is a source of Cre-LoxP recombination system: Correct. Phage P1 contains the Cre recombinase enzyme that is used in the Cre-LoxP system for gene manipulation, such as conditional knockout in genetically modified organisms.
- (4) Phage ΦX174 has single-stranded circular DNA genome: Correct. ΦX174 is a well-known bacteriophage with a single-stranded circular DNA genome, used in early studies of molecular genetics.
Information Booster:
- Phage M13 is commonly used in phage display techniques, where its single-stranded DNA genome is used to clone and display proteins.
- Phage Mu is a transposable element that inserts itself into bacterial DNA, making it useful for generating random mutations.
- The Cre-LoxP recombination system is used for gene editing, enabling precise genetic modifications by excising or inserting specific DNA sequences in a controlled manner.
- The ΦX174 phage was the first organism whose complete genome was sequenced, providing foundational knowledge in molecular biology.
