Correct option is B
Explanation-
Pichia pastoris is a species of methylotrophic yeast commonly used as an expression system for recombinant proteins, especially for biopharmaceutical production.
Statement A: It produces large amount of recombinant protein. - Correct
Pichia pastoris is known for its high cell density fermentation capability and strong, inducible promoters (e.g., AOX1), which allow high yields of recombinant proteins.
Statement B: It has the property of secreting proteins into the medium. - Correct
It can be genetically engineered to secrete proteins into the culture medium, simplifying purification and improving product recovery.
Statement C: It allows the formation of disulphide bonds similar to those in humans. - Correct
As a eukaryotic organism, Pichia pastoris supports post-translational modifications like disulfide bond formation, which are important for proper folding and activity of many human proteins.
Statement D: It carries out protein glycosylations identical to those found in humans. - Incorrect
Pichia pastoris does perform glycosylation, but not identical to humans. It adds high-mannose-type glycans, which can be immunogenic. Human-like glycosylation requires further genetic engineering (glycoengineering). Hence, without modification, its glycosylation is not human-like.
Correct Answer: Option b – A, B and C only
