Correct option is C
Explanation-
Option a : ddNTPs : Chain termination - CORRECT
ddNTPs (dideoxynucleotides) lack a 3’-OH group, which prevents the addition of further nucleotides during DNA synthesis. This property is utilized in Sanger sequencing to terminate DNA chains at specific bases.
Option b : South Western blot : Physical interaction between DNA and proteins - CORRECT
South Western blot is a hybrid technique combining Southern and Western blotting. It detects DNA-binding proteins by using labeled DNA probes. Hence, it studies the interaction between proteins and DNA.
Option c : 5’ → 3’ exonuclease activity : Proofreading polymerase for PCR - INCORRECT
Proofreading activity in DNA polymerases refers to 3’ → 5’ exonuclease activity (removing wrongly incorporated nucleotides). 5’ → 3’ exonuclease activity is involved in functions like removing primers or processing Okazaki fragments. In PCR, proofreading is due to 3’ → 5’ exonuclease, not 5’ → 3’.
Option d : Yeast two hybrid system : Interaction between proteins - CORRECT
Yeast two-hybrid system is used to detect protein-protein interactions.
Final Answer: Option c is incorrectly matched that makes it a correct answer.
