The critical micellar concentration (CMC) of a detergent is 5mM. choose the option that uses the minimum amount of detergent that can be used for cell lysis and is least likely to denature soluble proteins?

Correct option is C

Explanation:

• Critical Micellar Concentration (CMC) is the concentration above which detergent molecules start forming micelles, which is necessary for solubilizing membranes and lysing cells.

• The minimum amount of detergent that can be used for cell lysis is typically just above the CMC. Using a detergent concentration significantly below the CMC (like 2.5 mM) would be insufficient for effective lysis.

• The concentration of 6 mM is just above the CMC (5 mM) and is ideal for efficiently solubilizing the membrane without the excessive detergent that could denature proteins.

Information Booster:

1. The CMC is the concentration at which detergents start to form micelles and are effective at solubilizing cell membranes.

2. A detergent concentration of 6 mM is just slightly above the CMC, which ensures efficient membrane lysis while minimizing the risk of denaturing soluble proteins.

3. A detergent concentration much higher than the CMC may result in excessive micelles, which can disrupt protein structures and lead to denaturation.

4. 2.5 mM would be insufficient to effectively lyse cells as it is below the CMC, meaning the detergent would not form micelles effectively.

5. 10 mM is above the CMC and could be excessive, leading to a higher risk of protein denaturation.

Additional Information:

• 0.5 mM: This is too low and will likely not be effective in solubilizing membranes for cell lysis.

• 10 mM: While it exceeds the CMC, it can be excessive for cell lysis, increasing the chances of protein denaturation.

• 6 mM: This concentration is the best choice as it is above the CMC and sufficient for efficient lysis, with minimal denaturation risk.

Thus, 6 mM is the best choice for minimal detergent use and reduced risk of denaturation.