In the context of signaling, the enzyme protein kinase C (PKC) depends on multiple molecules for its complete activation. This activation depends on the presence of:A. Phosphatidylserine at the inner leaflet of the plasma membrane.B. Ca²⁺C. Phosphatidylethanolamine at the inner leaflet of the plasma membrane.D. Diacylglycerol present in the inner leaflet of the plasma membrane.Which one of the following options represents the combination of all correct molecules?

Correct option is D

Protein Kinase C (PKC) is a family of enzymes involved in signal transduction pathways. PKC activation depends on:

Evaluating the Statements

Calcium ions ( $Ca^{2+}$ ): Required for conventional PKCs to translocate to the plasma membrane.
Phosphatidylserine (PS): A negatively charged phospholipid found on the inner leaflet of the plasma membrane, which helps anchor PKC.
Diacylglycerol (DAG): A lipid second messenger that directly activates PKC at the plasma membrane.

Statement A: "Phosphatidylserine at the inner leaflet of the plasma membrane."
- Correct.
- Phosphatidylserine (PS) is essential for PKC membrane localization and activation.
Statement B: "Ca²⁺."
- Correct.
- Calcium is required for conventional PKCs, assisting in membrane recruitment.
Statement C: "Phosphatidylethanolamine at the inner leaflet of the plasma membrane."
- Incorrect.
- Phosphatidylethanolamine (PE) is not directly involved in PKC activation.
Statement D: "Diacylglycerol present in the inner leaflet of the plasma membrane."
- Correct.
- Diacylglycerol (DAG) is a crucial activator of PKC, generated from PIP₂ hydrolysis

A, B, and C