​Given below are terms related to genome-editing tools in Column A and their features in Column B:​

​Which one of the following options is the most appropriate match between terms of Column A and Column B?​

Correct option is B

Solution:

1. ZFN (Zinc Finger Nucleases) matches with iii: Focus of Zinc finger DNA binding domain with endonuclease domain of FokI restriction enzyme. ZFNs consist of zinc fingers that specifically bind to the DNA sequence and an endonuclease that cuts the DNA.

2. Meganuclease matches with i: Homing endonuclease (I-SceI). Meganucleases are large, highly specific endonucleases, and I-SceI is a type of homing endonuclease.

3. CRISPR/Cas 9 matches with iv: Target specificity using guide RNA. The CRISPR/Cas9 system uses guide RNA to target specific DNA sequences for editing.

4. TALEN (Transcription Activator-Like Effector Nucleases) matches with ii: Repeat of -35 amino acid length, each amino acid binding a specific DNA base in the target sequence. TALENs use a series of repeats that bind specific DNA bases and cleave DNA at the target site.

Hence, the correct answer is:

Option 2:

• A - iii, B - i, C - iv, D - ii