Given below are few terms related to bioinformatics resources (Column X) and their functions/Applications ( Column Y)

Column X Resources		Column Y Functions/applications
A.	TrEML	I.	Analysis of recombination frequencies between molecular markers
B.	TBLASTN	II.	Database of protein sequences
C.	SCOP	III.	Comparison of amino acid sequence against nucleotide sequence database translated in all six reading frames
D.	Joint Map	IV.	Manually curated structural classification of proteins

Which one of the following options represents all correct matches between Column X and Column Y?

​Which one of the following combinations of enzymes used for cloning a linear insert fragment into a digested plasmid vector would have the least probability of generating self-ligated vectors in a cloning experiment following complete digestion of all vector molecules and no further enzymatic treatment of the vector?​

​For the template sequence given below, which one of the following combination of primers can hypothetically be used to amplify the target region (Ignore Tm & length parameters for the primers)? 5'- A T C G A C T A G NNNNNNNNNNNNNNNNN C C T A A T G C A G - 3'​

In recent decades, the use of genetic markers has allowed the rapid introgression and selection of desired breeding stocks in advance generations. In this regard, following statements are given:

A. AFLP markers can discriminate between homozygote and heterozygote genotypes.

B. Microsatellites (e.g. SSR) are capable of detecting higher level of polymorphism than RFLP.

C. SNPs are more prevalent in the coding regions of the genome.

D. SNP markers are the most suitable markers for both foreground and background selection.

Which one of the following combination of the above statements is correct?

​A researcher raised antibodies against sheep red blood cells (SRBCs) and purified the IgG fraction. Some of the IgG antibodies were then subjected to enzymatic digestion to have Fab, Fc and F(ab')₂ fractions. He placed each preparation in a separate tube (1 to 3), labeled the three tubes to indicate their contents, and incubated them on ice. After a while he noticed that the label on two of the tubes (1 and 2) had gotten erased. He did a test for tube 1 and found that the preparation in the tube agglutinated SRBCs but did not lyse them in presence of complement. Which preparation was in tube 1?​

Sequencing of the human genome has identified 67 putative gene encoding K⁺ channels. To characterize the function of one of these genes, a researcher would typically carry out the following experiments:

A. in vitro transcription of the cloned cDNA for this gene in a cell-free system to produce the corresponding mRNA
B. Injecting above mentioned mRNA into frog oocytes
C. Measuring channel-protein activity using a patch-clamping technique
D. Knocking out the gene encoding the homolog of this channel in frog oocytes and measuring channel activity using a patch-clamping technique

Choose the correct combination of experiments that would help in the characterization of the K⁺ channel.

​Which one of the following statements about Cre-mediated site-specific recombination at loxP sites is INCORRECT?​

​Heating of some nucleic acids shows an increase in the absorbance at 260 nm (A260) typified by the plot . The sharp transition midpoint is defined as melting temperature (Tm). Which one of the following nucleic acid samples is NOT expected to generate such a typical profile upon heating of its solution?​

A.
5'- AGTAGTATCAACTATCATGA-3'
3'- TCATCATAGTTGATAGTACT-5'

B.
5'- GACGTGCAGGTGCGGAGGTC-3'
3'- CTGCACGGTCCACCTCCAG-5'

C.
5'- TACGATGCACATGCTTGGA-3'
3'- ATGCTACGTGTACGAACCTG-5'

D.
5'- GAACGCTACGTTGCGATCCG-3'
3'- CTTGCATGCAACGCTAGGC-5'

Arrange the DNA fragments (A to D) in the order of decreasing melting temperature.

Pichia pastoris is a good host for producing human proteins for therapeutic use. Given below are some statements on the reasons for its utility.

A. It produces a large amount of recombinant protein.
B. It has the property of secreting proteins into the medium.
C. It allows the formation of disulphide bonds similar to those in humans.
D. It carries out protein glycosylations identical to those found in humans.

Which one of the following options represents a combination of correct statements?

Inverse PCR is performed for site-directed mutagenesis with complementary primers (having the desired mutation) using a plasmid having the cloned gene as template. The following statements were made regarding the above experiment.

A. PCR is followed by transformation of bacterial cells directly with the reaction mixture. A large number of the transformants will have the wild type gene.
B. The PCR mixture is treated with Dpn I and then used to transform bacterial cells. Most of the transformants will have the mutant gene.
C. PCR is followed by transformation of bacterial cells directly with the reaction mixture. None of the transformants will have the mutant gene.
D. The PCR mixture is treated with Dpn I and then used to transform bacterial cells. Half of the transformants will have the mutant gene.

Which one of the following options represents a combination of all correct statements?