Following statements were made for the production of cisgenic plants:

A. In cisgenics, the donor sequence does not necessarily replace the native gene sequence but is added to the recipient species.
B. Cisgenic plants might contain DNA sequences such as T-DNA borders from the plasmid vector.
C. Insertion of a cisgene may result in a gene mutation at the site of insertion similar to that of transgenics.
D. With regard to the species gene pool, cisgenics does not alter the gene pool of the recipient species.
E. Both cisgenesis and transgenesis can use the same DNA transformation methods to introduce the respective gene constructs into the recipient plants.

Which one of the following options represents the combination of all correct statements?

Correct option is D

The correct answer is: (4) A, B, C, D and E

Explanation:

• Statement A is correct. In cisgenics, the donor sequence is added to the recipient species but does not replace the native gene. This makes cisgenics a more natural genetic modification since the sequence is similar to the native gene.

• Statement B is correct. Cisgenic plants might contain DNA sequences, such as T-DNA borders from the plasmid vector used for transformation, which is common in both cisgenesis and transgenesis.

• Statement C is correct. Just like transgenics, the insertion of a cisgene can lead to mutations at the insertion site in the recipient species. This is a common issue in both cisgenics and transgenics.

• Statement D is correct. Cisgenics does not alter the gene pool of the recipient species, as the gene inserted is from the same species or a closely related species, meaning no foreign DNA from outside the species is introduced.

• Statement E is correct. Cisgenesis and transgenesis can both use the same DNA transformation methods, such as Agrobacterium-mediated transformation or biolistic methods, to introduce genes into recipient plants.

Information Booster:

1. Cisgenics involves adding a gene from the same species or a closely related species without altering the genetic pool.

2. T-DNA borders are part of the transformation vector used in both cisgenics and transgenics.

3. Both cisgenesis and transgenesis can result in mutations at the insertion site.

4. Cisgenics does not alter the genetic diversity of the species, making it more akin to traditional breeding techniques.

5. DNA transformation techniques used in cisgenesis and transgenesis are largely the same.