​Figure A represents the sites for NcoI (N) in a binary vector pCSIR2023. The vector is 16500 bp in size. Figure B represents the fragments observed when the vector is either digested with NcoI (N) or double-digested with HindIII and NcoI (H+N). The intensity of the fluorescence of the 453 bp fragment is double that of the 516 bp fragment

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Which one of the following statements regarding the numbers and location(s) of HindIII sites is correct?

Correct option is B

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Explanation:

Analysis of HindIII Sites in the Vector:

• NcoI and HindIII Digestion:
• NcoI digestion generates fragments such as 10789 bp, 10336 bp, and 1567 bp, and when NcoI and HindIII are combined, different fragments appear, such as 516 bp and 453 bp. These patterns help in locating the HindIII sites.
• HindIII Sites:
• In the double-digested vector, you can see the distinct fragment sizes, and from the pattern, you observe that one of the HindIII sites corresponds to a fragment size of ~1.6 Kb.
• This suggests that one of the HindIII sites is indeed located in the ~1.6Kb fragment generated by NcoI digestion, which matches the digestion pattern for Option 2.

• Three HindIII sites are present in the vector as observed from the fragment patterns.
• One of the HindIII sites is located in the ~1.6Kb fragment generated by NcoI digestion. This is consistent with the analysis of the fragment sizes in Figure B.