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​Which one of the following statements is correct in the context of erythromycin-mediated inhibition of protein synthesis in bacteria?​
Question

Which one of the following statements is correct in the context of erythromycin-mediated inhibition of protein synthesis in bacteria?

A.

Erythromycin inhibits protein synthesis at the step of elongation, but it nonetheless allows translation of the first few codons.

B.

Erythromycin inhibits protein synthesis at the step of elongation, and it prevents formation of even the first peptide bond.

C.

Erythromycin inhibits formation of the translation initiation complex

D.

Erythromycin is toxic to bacteria because it results in initiation of protein synthesis with elongator tRNAs.

Correct option is A

Erythromycin is a macrolide antibiotic that inhibits bacterial protein synthesis by targeting the 50S ribosomal subunit. Its specific mechanism of action involves:

  1. Binding to the 23S rRNA of the 50S subunit, which blocks the exit tunnel of the growing polypeptide chain.
  2. Preventing elongation by inhibiting peptide bond formation and translocation of the ribosome along the mRNA.
  3. Allowing translation initiation to occur, meaning that the ribosome can start translation and produce a few amino acids before elongation is blocked.

Thus, translation of the first few codons occurs before elongation is inhibited, making Option 1 the correct answer.

Analysis of Other Options:

  • Option 2 (Incorrect )

    • The statement suggests that erythromycin prevents formation of even the first peptide bond, which is incorrect.
    • Erythromycin does not inhibit initiation; it only affects elongation after a few codons have been translated.
  • Option 3 (Incorrect )

    • Erythromycin does not inhibit the formation of the translation initiation complex.
    • Initiation occurs normally, and translation starts, but elongation is blocked after a few codons.
  • Option 4 (Incorrect )

    • The statement is incorrect because erythromycin does not cause faulty initiation with elongator tRNAs.
    • Instead, it blocks the elongation step of protein synthesis.

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