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    Following statements were made about mitochondria: A. The D loop of the mitochondrial genome is required for replication, but not for the regulation o
    Question

    Following statements were made about mitochondria:
    A. The D loop of the mitochondrial genome is required for replication, but not for the regulation of transcription.
    B. The L strand of mitochondrial genome possesses more cytosine.
    C. In plants, most mitochondrial tRNAs are encoded by the nuclear genome and then imported into the mitochondrion.
    D. Cycloheximide inhibits protein synthesis by mitochondrial ribosomes, but does not affect eukaryotic cytosolic ribosomes.
    E. Some organisms have been found to carry linear mitochondrial DNA.

    Which one of the following options represents a combination of the correct statements?

    A.

    A, B, C

    B.

    B, D, E

    C.

    A, C, D

    D.

    B, C, E

    Correct option is D

    Explanation-

    Statement A - The D loop of the mitochondrial genome is required for replication, but not for the regulation of transcription   - Incorrect
    The D-loop does play a role in both replication and transcription. It contains the origin of replication and the promoters for transcription. So, saying it is not involved in transcription regulation is false.

    Statement B -The L strand of mitochondrial genome possesses more cytosine  - Correct
    The L (Light) strand is cytosine-rich, hence lighter than the G-rich H (Heavy) strand. This statement is true.

    Statement C - In plants, most mitochondrial tRNAs are encoded by the nuclear genome and then imported into the mitochondrion  -  
    Correct
    In plant mitochondria, many tRNA genes are missing. So, the nucleus encodes these tRNAs, and they are imported into mitochondria. This is true.

    Statement D - Cycloheximide inhibits protein synthesis by mitochondrial ribosomes, but does not affect eukaryotic cytosolic ribosomes   -  Incorrect
    This is reversed. Cycloheximide inhibits cytosolic (80S) ribosomes. It does not inhibit mitochondrial (prokaryotic-like, 70S) ribosomes. So this is false.

    Statement E - Some organisms have been found to carry linear mitochondrial DNA  -  Correct
    Yes, linear mitochondrial DNA has been observed in some fungi, protozoa, and yeasts. This statement is true.

    Thus, the correct combination is: B, C, E
    Hence, correct option is d - B, C, E







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