Which of the following tests is used for fat estimation?

Correct option is D

✅ (d) Ethanol emulsion test:
· This test detects fats by mixing the sample with ethanol, which dissolves lipids.
· Upon adding water, the lipids precipitate out and form a milky white emulsion, confirming fat presence.
· This simple qualitative test is widely used to estimate fat content in food samples.
❌ (a) Iodine solution test: Used to detect the degree of unsaturation in fats but not fat quantity. ❌ (b) Benedict’s test: Used to detect reducing sugars in carbohydrates. ❌ (c) Biuret test: Used to detect proteins based on peptide bonds.