​What is the net charge of the peptide Tyr-Val-Arg at pH 5.0?
The pK_as of alpha amino and carboxyl groups are 9.6 and 2.3, respectively. The pK_as of Tyr and Arg side chains are 10.46 and 12.48, respectively.​

Correct option is A

Explanation-

Given pKa values:
α-amino group (N-terminus): pKa = 9.6
α-carboxyl group (C-terminus): pKa = 2.3
Tyrosine (Tyr) side chain –OH: pKa = 10.46
Arginine (Arg) side chain –NH₃⁺: pKa = 12.48

Step-by-step Analysis at pH = 5.0:
1. N-terminal amino group (pKa = 9.6):
              pH 5.0 < pKa → Protonated → +1 charge
2. C-terminal carboxyl group (pKa = 2.3):
              pH 5.0 > pKa → Deprotonated → –1 charge
3. Tyrosine side chain (pKa = 10.46):
              pH 5.0 < pKa → Protonated → neutral (–OH group not ionized)
4. Arginine side chain (pKa = 12.48):
              pH 5.0 < pKa → Protonated → +1 charge
Total net charge:
N-terminal (α-NH₃⁺)              :  +1
C-terminal (–COO⁻)               :  –1
Tyrosine side chain (–OH)      :   0
Arginine side chain (–NH₃⁺)   :  +1
                                       Total   +1

Final Answer:
Option a : 1.0