The following statements describe a few basic features of the interferometric reflectance imaging sensor used as a biosensing platform:

A. This biosensing platform is capable of high-throughput multiplexing of protein-protein, protein-DNA and DNA-DNA interactions.
B. The sensing surface is prepared by robotic spotting of biological probes that are immobilized on functionalised Si/SiO2 substrate.
C. As biomass accumulates on the substrate surface, a change in the interferometric signature occurs and the change can be correlated to a quantifiable mass.
D. Using this technique, picometer changes in biomass may be detected.

Which one of the following options represents the combination of all correct statements?

Correct option is A

Explanation:

Statement A: This biosensing platform is capable of high-throughput multiplexing of protein-protein, protein-DNA and DNA-DNA interactions.
IRIS technology uses interference of light to detect biomolecular interactions on a surface. It is known for its ability to monitor multiple types of interactions simultaneously, including protein-protein, protein-DNA, and DNA-DNA interactions, in a high-throughput manner. This statement is correct.
Statement B: The sensing surface is prepared by robotic spotting of biological probes that are immobilized on functionalized Si/SiO₂ substrate.
IRIS typically involves a silicon substrate with a thin silicon dioxide (SiO₂) layer, which is functionalized to immobilize biological probes. Robotic spotting is a common method to precisely place these probes on the surface for multiplexed detection. This statement aligns with the standard preparation of IRIS sensors and is correct.
Statement C: As biomass accumulates on the substrate surface, a change in the interferometric signature occurs and the change can be correlated to a quantifiable mass.
IRIS works by detecting changes in the interference pattern of light reflected from the sensor surface. When biomass (e.g., proteins or DNA) binds to the surface, it alters the optical path length, causing a measurable change in the interferometric signature. This change can indeed be correlated to the mass of the accumulated biomass, making this statement correct.
Statement D: Using this technique, picometer changes in biomass may be detected.
IRIS is highly sensitive and can detect small changes in the optical path length, often on the order of nanometers, due to biomass accumulation. However, picometer (10⁻¹² m) resolution is extremely small—far beyond the typical sensitivity of IRIS, which is more commonly in the nanometer (10⁻⁹ m) range for height changes or mass detection. Detecting picometer changes would require extraordinary precision that is not typically associated with IRIS in practical biosensing applications. This statement is likely incorrect.

So. Statement A ,B ,and C are correct .