Correct option is D
The fed-batch fermentation process is a biotechnological process where nutrients are added in intervals or continuously during the fermentation process, without removing any culture liquid. In fed-batch mode, the volume of the culture medium increases over time because fresh nutrients are added, and this results in an increase in viable cell count.
Graph D represents the correct profile for a fed-batch fermentation because:
- The solid line (viable cell count) shows a continuous increase after a lag phase, indicating sustained microbial growth due to nutrient addition.
- The dotted line (volume of media) shows an increase over time, which matches the characteristic of fed-batch fermentation, where fresh nutrients are continuously or periodically added, leading to a rise in the culture volume.
Information Booster:
Fed-batch fermentation:
- Fresh nutrients are added during the fermentation process.
- There is no removal of culture liquid during the process.
- The volume of the fermentation medium increases with time as fresh media is added.
- This method allows for the control of nutrient levels, which can help in optimizing product formation and extending the growth phase of microorganisms.
Batch vs. Fed-batch fermentation:
- In batch fermentation, all nutrients are provided at the start, and no further addition is made. The volume remains constant.
- In fed-batch fermentation, nutrients are added over time, and the volume increases, leading to continued growth of the cells.
