Match the columns.

Correct option is C

The correct answer is (c) i-d, ii-c, iii-b, iv-a.
• i. Helicase - d. Opens up the DNA double helix
Helicase is an enzyme that unwinds the DNA double helix by breaking the hydrogen bonds between the nucleotide pairs, creating two single-stranded DNA templates.
• ii. RNA primase - c. RNA primer synthesis
RNA primase synthesizes short RNA primers on the lagging strand during DNA replication. These primers provide the starting point for DNA polymerase to begin nucleotide addition.
• iii. DNA polymerase - b. Nucleotide polymerisation
DNA polymerase is responsible for adding nucleotides to the growing DNA strand during replication, ensuring that the DNA sequence is accurately copied.
• iv. DNA ligase - a. Joins the 3’ end of the new DNA fragment to the 5’ end of the previous one
DNA ligase seals the gaps between Okazaki fragments on the lagging strand, creating a continuous DNA strand by forming phosphodiester bonds between adjacent nucleotides.
This matching reflects the key roles each enzyme/protein plays in the DNA replication process.