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In which of the following technique, DNA from different sources are isolated and digested with restriction endonuclease?
Question

In which of the following technique, DNA from different sources are isolated and digested with restriction endonuclease?

A.

RFLP

B.

AFLP

C.

SSR

D.

RAPD

Correct option is A


The correct answer is (a) RFLP, which stands for Restriction Fragment Length Polymorphism. This technique involves using restriction enzymes to cut DNA into fragments, which can then be separated by gel electrophoresis based on their size. This method is particularly useful for genetic mapping, analyzing the genetic diversity, and identifying genetic markers. RFLP relies on the presence of specific recognition sites for restriction endonucleases in DNA sequences, which will vary among different genetic sources, leading to fragments of varying lengths.

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