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    Human prostate cancer cells expressing urokinase Plasminogen Activator (uPA) readily metastasize when injected into experimental animals. A scientist
    Question

    Human prostate cancer cells expressing urokinase Plasminogen Activator (uPA) readily metastasize when injected into experimental animals. A scientist genetically modified prostate cancer cells and injected them into experimental animals. The possible observations are listed below:
    A. Overexpressing a mutant version of the uPA protein that does not bind to its receptor led to tumour formation but reduced metastasis.
    B. Overexpressing a mutant version of the uPA protein that does not bind to its receptor led to enhanced tumour formation as well as metastasis.
    C. Overexpressing a secreted version of the uPA receptor led to tumour formation but reduced metastasis.
    D. Overexpressing a secreted version of the uPA receptor reduced tumour formation as well as metastasis.
    Which of the following options represents the combination of all correct observations?

    A.

    A and C

    B.

    B and D

    C.

    A and D

    D.

    B and C

    Correct option is A

    Answer: A and C (option a)

    Explanation


    -uPA promotes invasion/metastasis by binding the cell-surface uPA receptor (uPAR) to focus proteolytic activity and trigger signaling for cell migration.
    -A mutant uPA that cannot bind uPAR will not promote receptor-dependent invasion, so primary tumour formation (driven by other oncogenic events) can still occur while metastasis is reduced.
    -A secreted (soluble) uPAR acts as a decoy that sequesters uPA and prevents uPA–uPAR interactions at the cell surface, so it reduces metastasis but does not necessarily block tumour formation.

    Information Booster

    -uPA–uPAR interaction localizes plasminogen activation to the cell surface, generating plasmin that degrades extracellular matrix and activates other proteases—key for invasion and metastasis.
    -Decoy receptors or receptor-binding mutants commonly reduce invasive behaviour while leaving proliferation (tumour growth) largely unaffected unless the pathway also controls growth signals.

    Additional Knowledge — why the other options are wrong

    -B (mutant uPA that does not bind uPAR → enhanced tumour formation and metastasis): contradicts mechanism — loss of receptor binding should reduce invasion/metastasis, not enhance it.
    -D (secreted uPAR reduces tumour formation as well as metastasis): unlikely because soluble uPAR blocks uPA-mediated invasion but typically does not eliminate primary tumour growth unless uPA–uPAR is also essential for proliferation in that model (not implied here).

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