Arrange the steps for the estimation of Hb through Sahli’s Hemoglobinometer in the correct order: A. Ensure cleanliness and dryness of the hemoglobinometer tubes and pipette. B. Fill the hemoglobinometer tube with N/10 HCl up to its lowest mark (2% or 10%) using a dropper. C. Draw blood up to the mark in the Sahli’s pipette (20 µl), wipe off excess blood, and transfer it to the N/10 HCl in the hemoglobin tube. D. Allow it to sit for 10 minutes for complete hemoglobin to hematin conversion. E. Add distilled water drop by drop, stirring until the color matches the standard glass of the comparator. F. Read the hemoglobin concentration at the lower meniscus, providing the concentration in 100 ml of blood.

Correct option is A

The correct sequence involves ensuring cleanliness, filling the hemoglobinometer with N/10 HCl, drawing the blood, and allowing the reaction to occur for proper conversion to hematin. Afterward, distilled water is added to adjust the color to match the comparator, and the reading is taken at the lower meniscus to determine hemoglobin concentration.
Explanation of each option:
· (a) ABCDEF – Correct. This is the proper sequence of steps for estimating hemoglobin using Sahli’s Hemoglobinometer.
· (b) AFDCFB – Incorrect. The order is not followed correctly, especially with step D (waiting for hemoglobin conversion).
· (c) FEADCB – Incorrect. The steps are out of order, particularly with the wrong sequence of adding distilled water and allowing for conversion.
· (d) DCEFBA – Incorrect. This sequence disrupts the crucial step of ensuring cleanliness and the proper mixing of blood with HCl.