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    ABA (Abscisic Acid) plays an important role in plant response to water stress. In the table below, column X represents some of the important enzymes i
    Question

    ABA (Abscisic Acid) plays an important role in plant response to water stress. In the table below, column X represents some of the important enzymes in ABA biosynthesis/degradation pathways, while column Y summarizes the major function of these enzymes.

    Column X (Enzymes)

    Column Y (Functions)

    A. 9-cis-epoxycarotenoid dioxygenase

    i. Involved in the oxidative pathway of ABA catabolism

    B. cytochrome P450 monooxygenase (CYP707A3)

    ii. Production of a sugar – conjugated form of ABA

    C. ABA glucosyltransferase

    iii. Xanthoxin production

    D. β – glucosidase

    iv. Releases ABA from its sugar – conjugated form

    Choose the option showing the correct match between column X and column Y:

    A.

    A-iv, B-ii, C-i, D-iii

    B.

    A-iii, B-i, C-ii, D-iv

    C.

    A-ii, B-iii, C-iv, D-i

    D.

    A-i, B-iv, C-iii, D-ii

    Correct option is B

    ABA (Abscisic Acid) is a crucial plant hormone that regulates stress responses, particularly indrought conditions. It is synthesized and degraded through specific enzymatic pathways:

    1. (A)9-cis-epoxycarotenoid dioxygenase→ Xanthoxin production (iii)

      • 9-cis-epoxycarotenoid dioxygenase (NCED)is akey enzyme in ABA biosynthesis.
      • It catalyzes the conversion of9-cis-epoxycarotenoids to xanthoxin, an important ABA precursor.
      • Correct match: A-iii
    2. (B) Cytochrome P450 monooxygenase (CYP707A3) → Oxidative ABA catabolism (i)

      • CYP707A3 is amajor enzyme involved in ABA degradation.
      • Itoxidizes ABA, leading to itsinactivation.
      • Correct match: B-i
    3. (C) ABA glucosyltransferase → Production of a sugar-conjugated form of ABA (ii)

      • ABA glucosyltransferase catalyzes theaddition of glucoseto ABA, formingABA-glucose conjugates(ABA-GE).
      • This is a storage/inactive form of ABA.
      • Correct match: C-ii
    4. (D) β-glucosidase → Releases ABA from its sugar-conjugated form (iv)

      • β-glucosidase breaks downABA-glucose conjugates (ABA-GE)to releasefree ABA.
      • This reactivates ABA understress conditions.
      • Correct match: D-iv

    Information Booster

    1. ABA Biosynthesis Pathway:

      • 9-cis-epoxycarotenoid dioxygenase (NCED)is arate-limiting enzymein ABA biosynthesis.
      • Xanthoxin is converted intoABA aldehyde, which is then oxidized to form ABA.
    2. ABA Degradation Pathway:

      • ABA is degraded primarily viaoxidation, catalyzed byCYP707A3, reducing ABA levels duringstress recovery.
    3. ABA Conjugation & Release:

      • ABA can be stored in aninactiveform as ABA-glucose ester (ABA-GE), catalyzed byABA glucosyltransferase.
      • Under stress,β-glucosidasehydrolyzes ABA-GE,releasing free ABA, which reactivates the stress response.
    4. Role of ABA in Drought Stress:

      • ABAinduces stomatal closuretoreduce water loss.
      • It triggersgene expressionthat enhances drought resistance.
    5. CYP707A3 and ABA Homeostasis:

      • Mutations inCYP707A3lead tohigher ABA levels, making plants more drought-resistant butdelayed in stress recovery.
    6. Commercial Applications of ABA:

      • ABA is used inagricultureto enhance drought resistance in crops.
      • Synthetic ABA analogs are being explored for improving plantwater-use efficiency.

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