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Which one of the following is a correct combination of four-carbon intermediates formed during C4 photosynthesis in plants?
Question

Which one of the following is a correct combination of four-carbon intermediates formed during C4 photosynthesis in plants?

A.

Malate and Aspartate

B.

Aspartate and Alanine

C.

Phosphoenolpyruvate and Oxaloacetate

D.

Alanine and Pyruvate

Correct option is A

C4 photosynthesis is a specialized pathway that helps plants efficiently fix CO₂ under high light intensity and water stress conditions. It involves the Hatch-Slack Pathway, where CO₂ is initially fixed into a four-carbon compound before being transported to bundle sheath cells for the Calvin cycle. 

Steps of C4 Photosynthesis:

  1. Carbon Fixation in Mesophyll Cells:

    • CO₂ is fixed by Phosphoenolpyruvate (PEP) to form Oxaloacetate (OAA) (a four-carbon compound) via the enzyme PEP carboxylase.
    • OAA is then converted into Malate or Aspartate, depending on the plant type.
  2. Transport to Bundle Sheath Cells:

    • Malate or Aspartate is transported to the bundle sheath cells, where CO₂ is released for the Calvin cycle.
  3. Regeneration of PEP:

    • The remaining three-carbon compound (Pyruvate or Alanine) is transported back to mesophyll cells and converted to PEP, allowing the cycle to continue.

Information Booster

  1. C4 Pathway Overview: A carbon-concentrating mechanism that enhances CO₂ availability for Rubisco, reducing photorespiration.
  2. C4 Intermediates: The major four-carbon intermediates include Oxaloacetate, Malate, and Aspartate.
  3. Enzymes Involved:
    • PEP Carboxylase → Fixes CO₂ into Oxaloacetate.
    • Malate Dehydrogenase → Converts Oxaloacetate into Malate.
    • Aspartate Aminotransferase → Converts Oxaloacetate into Aspartate.
  4. Types of C4 Plants:
    • NADP-ME Type: Uses Malate as the CO₂ carrier (e.g., Maize, Sugarcane).
    • PCK Type: Uses Aspartate as the CO₂ carrier (e.g., Panicum spp.).
  5. Efficiency of C4 Photosynthesis:
    • Higher water-use efficiency than C3 plants.
    • Found in tropical and arid environments.
  6. Comparison with CAM Plants:
    • Both use PEP carboxylation, but CAM plants store CO₂ at night to avoid water loss.
  7. C4 Evolution:
    • Evolved in grasses and some dicots to overcome photorespiration under low CO₂ and high oxygen conditions.

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