Which of the options correctly matches the proteins involved in transcription (Column A) with the DNA binding domains they carry (Column B)?
Explanation-
A. TFIIIA → Zinc finger (ii)
TFIIIA is a classical zinc finger protein.
B. MyoD → Helix-loop-helix (iii)
MyoD is a bHLH (basic helix-loop-helix) transcription factor.
C. Jun → Leucine zipper (iv)
Jun (part of AP-1) has a leucine zipper DNA-binding domain.
D. Cro → Helix-turn-helix (i)
Cro protein from lambda phage uses a helix-turn-helix motif to bind DNA.
So, the correct answer is option d -A-ii, B-iii, C-iv, D-i .
Which one of the following statements is NOT a correct feature of Escherichia coli RNA polymerase?
Which one of the following schematics depicts the potential relationship among the subunits Ilo, IIa, and IIb of RNA polymerase II?
Which one of the following regions of the target gene is NOT used for making an RNAi construct to knock down its expression?
All of the following statements about bacterial transcription termination are true EXCEPT:
The initiation of transcription is a complex process involving promoter recognition, conversion of the initiation complex from closed to open form, abortive initiation events, and finally promoter escape. The following statements are made regarding these steps in transcription initiation:
A. Promoter escape in bacteria is usually accompanied by the release of the sigma factor from the RNA polymerase holoenzyme complex.
B. Abortive initiation events in prokaryotes result in the formation of short transcripts ~10 nucleotides in length while such events in eukaryotes result in formation of transcripts ~75 nucleotides in length.
C. Promoter escape in eukaryotes is accompanied by the phosphorylation of the RNA polymerase large subunit on its C-terminal domain (CTD).
D. Promoter recognition in bacteria is governed by the sigma factor which binds to the -10 and -35 regions of the promoter followed by recruitment of the RNA Pol II core enzyme to form the holoenzyme.
Which one of the following options represents the combination of all correct statements?
Which of the options correctly matches the proteins involved in transcription (Column A) with the DNA binding domains they carry (Column B)?
In a study, researchers replaced the natural promoter of a gene with a synthetic promoter that contains a point mutation in the TATA box that prevents binding of the TATA-binding protein (TBP). The following outcomes would most likely result from this modification.
A. An mRNA will be generated with an alternate reading frame.
B. The mRNA will be transcribed by RNA polymerase I instead of RNA polymerase II.
C. Transcription may occur with a reduced efficiency.
D. Transcription may occur but will always result in the formation of a non-functional mRNA.
Which one of the following options represents the combination of all INCORRECT statements?
For expression of a gene of interest (Goi) and a green fluorescent protein (GFP) in mammalian cells, Goi and GFP must be expressed in a single mRNA, but translated independently. Which one of the following would be the structure of the expression construct?
(Pro - promoter; Enh - enhancer; IRES - internal ribosome entry site; pA - polyadenylation signal sequence)
The figure below depicts the feedback regulation in the biosynthesis pathway of three branched-chain amino acids (BCAA) - Leu, Val, and Ile, acting at three major steps catalyzed by enzymes AHAS, IPMS, and TD. The activity of AHAS is feedback regulated by the synergistic combination of Leu and Val. IPMS activity is regulated exclusively by Leu. Ile regulates TD activity, while Val can relax this feedback regulation on TD by Ile.
Which one of the following possibilities of BCAA pools is likely to occur in the RNAi knockdown of IPMS?
Which one of the following statements about RNA polymerase in eukaryotes is INCORRECT?
