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    Which of the following techniques was used to develop browning-free apple (Arctic apples)?
    Question

    Which of the following techniques was used to develop browning-free apple (Arctic apples)?

    A.

    Genome editing

    B.

    Cisgenesis

    C.

    RNAi

    D.

    Hybridization

    Correct option is C

    The correct answer is: (C) RNAi
    The browning-free Arctic apples were developed using RNA interference (RNAi) technology. This technique silences specific genes responsible for enzymatic browning in apples.
    In Arctic apples, the polyphenol oxidase (PPO) gene is suppressed, preventing the oxidation of phenolic compounds, which causes browning when the fruit is cut or bruised.
    Information Booster:
    • RNA interference (RNAi) is a gene-silencing technique that blocks the expression of specific mRNA, preventing the production of unwanted proteins.

    • Arctic apples retain their fresh appearance for a longer time, making them more appealing for consumers and reducing food waste.

    Knowledge Booster:
    • Genome editing: Genome editing techniques, such as CRISPR-Cas9, are used to directly modify DNA sequences.

    • Cisgenesis: involves transferring genes from the same or closely related species to improve traits.​

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