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    ​Which of the following 'R' gene products do not contain NBS-LRR domain?​
    Question

    Which of the following 'R' gene products do not contain NBS-LRR domain?

    A.

    RPS2 protein of Arabidopsis

    B.

    Xa1 protein of rice

    C.

    N protein of tobacco

    D.

    Mlo protein of barley

    Correct option is D

    Explanation-

    ‘R’ genes (Resistance genes) in plants encode resistance (R) proteins that detect specific pathogens and activate defense mechanisms.
    A common structure of many R proteins is the NBS-LRR domain:
           1.    NBS = Nucleotide Binding Site → involved in ATP/GTP binding and hydrolysis.
           2.    LRR = Leucine-Rich Repeat → involved in protein-protein interactions, especially recognizing pathogen effectors.
    These domains are cytoplasmic and part of a large class of R proteins called NBS-LRR proteins.

    Option a - RPS2 protein of Arabidopsis
    Full name: Resistance to Pseudomonas syringae 2
    Class: NBS-LRR
    Recognizes AvrRpt2, a type III effector protein from Pseudomonas syringae.
    Function: After recognition, RPS2 triggers a hypersensitive response (HR) to stop pathogen spread.
    It contains NBS-LRR domain 
    Option b -  Xa1 protein of rice
    Xa1 confers resistance to Xanthomonas oryzae pv. oryzae, the bacterial blight pathogen. It is found in rice, functions through recognition of bacterial effectors. It contains NBS-LRR domain 
    Option c -  N protein of tobacco
    Confers resistance to Tobacco Mosaic Virus (TMV). It belongs to TIR-NBS-LRR subclass (TIR = Toll/Interleukin-1 receptor-like domain). It activates localized cell death upon virus detection. It contains NBS-LRR domain .

    Option d - Mlo protein of barley 
    This is very different from others . It is not an NBS-LRR protein. It is a seven-transmembrane domain protein, localized in the plasma membrane. Resistance occurs via loss-of-function mutations in the Mlo gene. These mutations prevent the fungus Blumeria graminis (causing powdery mildew) from colonizing cells.
    Thus, Mlo-mediated resistance is recessive, unlike dominant NBS-LRR-based resistance.

    Final answer : option d - Mlo protein of barley

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