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    Which of the following is one of the proven techniques for virus elimination in plant multiplication?
    Question

    Which of the following is one of the proven techniques for virus elimination in plant multiplication?

    A.

    Shoot tip culture

    B.

    Grafting

    C.

    Leaf culture

    D.

    Nodal segment culture

    Correct option is A

    The correct answer is: (A) Shoot tip culture
    Explanation:
    Shoot tip culture is a widely used technique for virus elimination in plant propagation.
    This method involves the excision and in vitro cultivation of a very small portion (0.2–0.5 mm) of the shoot apex, which contains meristematic cells that are often free from viral infection.
    Since most plant viruses do not reach the meristematic region, growing a new plant from this uninfected tissue helps produce virus-free plants.
    Information Booster:
    • Why shoot tip culture is effective?

      • The meristematic region lacks vascular connections that transport viruses.

      • When combined with thermotherapy (heat treatment) or chemotherapy (antiviral chemicals), virus elimination becomes even more efficient.

    • Common crops where shoot tip culture is used:

      • Banana, potato, citrus, sugarcane, orchids, and strawberries.

    • Advantages of shoot tip culture:

      • Produces disease-free planting material.

      • Ensures genetic uniformity in clonal propagation.

      • Used in large-scale micropropagation programs.​

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