Correct option is D
What is a Test Cross?
A
test cross is a genetic tool used to
evaluate the genotype of an individual by
crossing it with a common "tester" parent.
The
tester is usually:
A
homozygous recessive line (in classical Mendelian genetics)
Or a
standard inbred line (in plant breeding programs)
�� Purpose of Test Crosses in Breeding:
To
assess combining ability, especially
General Combining Ability (GCA) and
Specific Combining Ability (SCA).
Used extensively in
line × tester analysis where
multiple lines are crossed with a
single tester.
Helps breeders identify which lines are good general combiners for traits like yield, disease resistance, etc.
�� Clarification of Other Options:
(a) Single crosses – ❌ A cross between
two specific inbred lines (A × B).
(b) Double crosses – ❌ A hybrid made by crossing
two single crosses: (A × B) × (C × D).
(c) Three-way crosses – ❌ Cross between a
single cross and another inbred: (A × B) × C.
(d) Test crosses – ✅ Crosses made using a
common tester, used for evaluating breeding lines.