Correct option is B
The correct preposition to use in the sentence is "on".
So, the complete sentence would be:
"My birthday is on 9th September."
"On" is used before dates.
For example, "on 9th September" specifies the exact date.
Other Prepositions:
"In" is used for months, years, centuries, and long periods (e.g., in September, in 2024).
"At" is used for precise times (e.g., at 5 PM).