There are various types of restriction endonucleases (RE) which are capable of cutting DNA either within or away from the recognition site.
Which of the following RE can cut DNA strands specifically within the recognition site?

Correct option is B

Correct Answer: (b)
Explanation:
Type II restriction endonucleases cut the DNA precisely within their specific recognition sites.
Type I and Type III enzymes cut at sites away from the recognition sequence.
Information Booster:
Type II REs are most useful in genetic engineering (e.g., EcoRI, HindIII).
They recognize palindromic sequences.
Type I and Type III require ATP for activity.
Type II works best in defined buffer conditions (Mg²⁺ dependent).
These enzymes generate sticky or blunt ends useful for cloning.
Additional Information (Incorrect Options):
(a), (c), and (d) include Type I/III, which do not cut within the recognition site.