Correct option is D
Detailed Explanation:
Let's carefully evaluate the potential outcomes of each of the strategies:
Strategy A: Increasing catalytic efficiency of the rate-limiting biosynthetic enzyme in the source organ
- This is helpful for increasing the synthesis of the metabolite M in the source organ, providing more metabolite for transport to the sink organ. Increasing the efficiency of the biosynthetic enzyme can increase the amount of ‘M’ produced and thus available for transport.
Strategy B: Increasing catalytic efficiency of the catabolic enzymes in the source organ
- This is counterproductive. If the catabolic enzymes (which break down the metabolite) are more efficient in the source organ, they will degrade ‘M’ more quickly, reducing the amount available for transport to the sink organ.
Strategy C: Generating knock-out of the transporter protein in the source organ
- This is counterproductive. Transporters are responsible for moving the metabolite from the source to the sink. Knocking out the transporter will prevent ‘M’ from being transported to the sink organ, thus reducing its accumulation in the sink.
Strategy D: Repression of the catabolic enzymes in the sink organ
- This is helpful. Repressing the catabolic enzymes in the sink organ will prevent the breakdown of ‘M’, leading to higher accumulation of ‘M’ in the sink organ.
Correct Match:
- A and D are the strategies that will increase the accumulation of metabolite M in the sink organ:
- A: Increases biosynthesis of ‘M’ in the source organ.
- D: Reduces the catabolic breakdown of ‘M’ in the sink organ.
