Correct option is D
Explanation-
φ×174 (phi X 174) is a bacteriophage (virus that infects bacteria), and it contains single-stranded (+) sense DNA. Its replication involves a replicative form (RF), which is a double-stranded DNA intermediate used for replication.
Statement A - "The single stranded φ×174 DNA is converted into a double-stranded replicative form (RF)" - CORRECT
When φ×174 infects E. coli, the single-stranded (+) DNA is first converted into a double-stranded replicative form (RF) by synthesizing the complementary (-) strand. This RF is used as a template for further replication and transcription.
Statement B - "Replication of double stranded replicative form results in the production of single stranded phages, about 50% of which are +ve sense phages and the remaining are –ve sense phages" - INCORRECT
Only +ve sense single-stranded DNA is packaged into progeny phages. The phage only produces + sense ssDNA, which is then encapsidated (enclosed in the protein coat). The (-) sense strand is not packaged; it's only a template.
Statement C - "Replication of the double stranded replicative form results in the production of only –ve sense phages" - INCORRECT
As stated above, only +ve sense strands are produced for packaging.
Statement D - "Replication of the double stranded replicative form results in the production of only +ve sense phages" - CORRECT
From the RF, multiple +ve sense ssDNA molecules are synthesized and packaged into new phage particles.
Final Answer -
Correct option : d (A and D).




which one of the following statements about the regulation by CI and Cro proteins is CORRECT?