In CRISPR-Cas9 technology, the RNA designed to find and bind to a specific sequence in the DNA is known as

Correct option is B

✅ (b) gRNA
· gRNA (guide RNA) is a synthetic RNA designed to guide the Cas9 enzyme to a specific DNA target sequence.
· It has a scaffold sequence that binds Cas9 and a spacer sequence complementary to the target DNA.
· gRNA-Cas9 complex introduces a double-strand break at the target site for genome editing.
· Central to the precision of CRISPR-Cas9 systems in biotechnology and genetic engineering.
❌ (a) mRNA
· mRNA (messenger RNA) carries genetic information from DNA to ribosomes for protein synthesis.
· Not involved in DNA targeting for CRISPR applications.
❌ (c) ScRNA
· ScRNA (small cytoplasmic RNA) is involved in protein secretion processes in eukaryotic cells.
· Not related to CRISPR-Cas9 targeting mechanisms.
❌ (d) U1RNA
· U1 snRNA is part of the spliceosome complex, essential for pre-mRNA splicing.
· It has no role in CRISPR-Cas9 guided DNA editing.