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​Following are marker enzymes that would be used to identify correct subcellular fractions.Column XColumn YENZYMEFRACTIONA. Lactate dehydrogenasei.Lys
Question

Following are marker enzymes that would be used to identify correct subcellular fractions.

Column X

Column Y

ENZYME

FRACTION

A.

Lactate dehydrogenase

i.

Lysosomes

B

Acid phosphatase

ii.

Microsomes

C.

Glucose-6-phosphatase

iii.

Cytosol

D.

Catalase

iv.

Peroxisomes

Which one of the following options correctly pairs the enzymes with the subcellular fractions?

A.

A-i, B-ii, C-iii, D-iv

B.

A-iv, B-iii, C-i, D-ii

C.

A-iii, B-i, C-ii, D-iv

D.

A-ii, B-iv, C-i, D-iii

Correct option is C

Lactate dehydrogenase (A):

  • This enzyme is primarily found in the cytosol, where it catalyzes the conversion of lactate to pyruvate.
  • Correct pair: A-iii (Cytosol).

Acid phosphatase (B):

  • Acid phosphatase is a lysosomal enzyme involved in breaking down phosphates in acidic environments.
  • Correct pair: B-i (Lysosomes).

Glucose-6-phosphatase (C):

  • This enzyme is found in the microsomes, particularly involved in glucose metabolism and present in the endoplasmic reticulum.
  • Correct pair: C-ii (Microsomes).

Catalase (D):

  • Catalase is a marker enzyme for peroxisomes, where it breaks down hydrogen peroxide into water and oxygen.
  • Correct pair: D-iv (Peroxisomes).

Information Booster:

Identification of Organelles:

  • Marker enzymes are essential tools in understanding the role of specific organelles. By measuring enzyme activity, we can identify and characterize various organelles in a cell.

Subcellular Fractionation:

  • Cell fractionation is a process where cells are disrupted, and their components are separated based on their size, density, and shape. Marker enzymes help identify these separated components.

Understanding Cellular Functions:

  • By knowing which enzymes are associated with which organelles, researchers can gain insights into cellular functions like metabolism, waste processing, and detoxification.

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